When NanoSim tries to fit the mixed statistical model, it performs a goodness of fit test (mostly for development purpose). When the fitted parameters fail to pass the test, it will output this warning. But normally the discrepancy is still very small, so the error rate and error patterns are close enough to the raw reads.
When NanoSim tries to fit the mixed statistical model, it performs a goodness of fit test (mostly for development purpose). When the fitted parameters fail to pass the test, it will output this warning. But normally the discrepancy is still very small, so the error rate and error patterns are close enough to the raw reads.