Closed xiekunwhy closed 3 months ago
[x] version of RNA-Bloom with java -jar RNA-Bloom.jar -version /Bio/User/software/anaconda3/envs/rnabloom/bin/rnabloom RNA-Bloom v2.0.0
java -jar RNA-Bloom.jar -version
[x] version of java with java -version
java -version
/Bio/User/software/anaconda3/envs/rnabloom/bin/java -version openjdk version "17.0.3-internal" 2022-04-19 OpenJDK Runtime Environment (build 17.0.3-internal+0-adhoc..src) OpenJDK 64-Bit Server VM (build 17.0.3-internal+0-adhoc..src, mixed mode, sharing)
source /Bio/User/software/anaconda3/bin/activate /Bio/User/software/anaconda3/envs/rnabloom;/Bio/User/software/anaconda3/envs/rnabloom/bin/rnabloom -revcomp-right -t 10 -ntcard -prefix R21051817_000 -left /state/partition1/WORK/Bio/Project/xiek/project/GDDXXXX_std_4/pipe/pipe3/02.mrna/04.assemble/denovoasm/R21051817_1.fq.gz -right /state/partition1/WORK/Bio/Project/xiek/project/GDDXXXX_std_4/pipe/pipe3/02.mrna/04.assemble/denovoasm/R21051817_2.fq.gz -outdir /state/partition1/WORK/Bio/Project/xiek/project/GDDXXXX_std_4/pipe/pipe3/02.mrna/04.assemble/denovoasm/R21051817_rnabloom -q 3 -Q 20 -c 1 -e 1 -bound 1000 -extend -stratum e0
Reducing redundancy in assembled transcripts... Parsed 0 overlap records in 0.011s before: 88,142 after: 88,142 ERROR: Error during redundancy reduction!
Best, Kun
Thanks Kun for reporting this! In this case, you can use transcripts.fa (instead of transcripts.nr.fa) as your assembly.
transcripts.fa
transcripts.nr.fa
Please report
[x] version of RNA-Bloom with
java -jar RNA-Bloom.jar -version
/Bio/User/software/anaconda3/envs/rnabloom/bin/rnabloom RNA-Bloom v2.0.0[x] version of java with
java -version
/Bio/User/software/anaconda3/envs/rnabloom/bin/java -version openjdk version "17.0.3-internal" 2022-04-19 OpenJDK Runtime Environment (build 17.0.3-internal+0-adhoc..src) OpenJDK 64-Bit Server VM (build 17.0.3-internal+0-adhoc..src, mixed mode, sharing)
source /Bio/User/software/anaconda3/bin/activate /Bio/User/software/anaconda3/envs/rnabloom;/Bio/User/software/anaconda3/envs/rnabloom/bin/rnabloom -revcomp-right -t 10 -ntcard -prefix R21051817_000 -left /state/partition1/WORK/Bio/Project/xiek/project/GDDXXXX_std_4/pipe/pipe3/02.mrna/04.assemble/denovoasm/R21051817_1.fq.gz -right /state/partition1/WORK/Bio/Project/xiek/project/GDDXXXX_std_4/pipe/pipe3/02.mrna/04.assemble/denovoasm/R21051817_2.fq.gz -outdir /state/partition1/WORK/Bio/Project/xiek/project/GDDXXXX_std_4/pipe/pipe3/02.mrna/04.assemble/denovoasm/R21051817_rnabloom -q 3 -Q 20 -c 1 -e 1 -bound 1000 -extend -stratum e0
Reducing redundancy in assembled transcripts... Parsed 0 overlap records in 0.011s before: 88,142 after: 88,142 ERROR: Error during redundancy reduction!
Best, Kun