Closed bede closed 1 year ago
% hostile dehost --fastq1 tests/data/h37rv_10.r1.fastq.gz --fastq2 tests/data/h37rv_10.r2.fastq.gz --out-dir test INFO: Using Bowtie2 INFO: Using cached human index (/Users/bede/Library/Application Support/hostile/human-bowtie2) Dehosting: 0%| | 0/1 [00:00<?, ?it/s]Exception occurred during executing command bowtie2 -x '/Users/bede/Library/Application Support/hostile/human-bowtie2' -1 'tests/data/h37rv_10.r1.fastq.gz' -2 'tests/data/h37rv_10.r2.fastq.gz' -k 1 --mm -p 10| tee >(samtools view -F 256 -c - > test/h37rv_10.r1.reads_in.txt)| samtools view --threads 5 -f 12 - | tee >(samtools view -F 256 -c - > test/h37rv_10.r1.reads_out.txt) | awk 'BEGIN{FS=OFS="\t"} {$1=int((NR+1)/2)" "; print $0}' | samtools fastq --threads 5 -c 6 -N -1 'test/h37rv_10.r1.dehosted_1.fastq.gz' -2 'test/h37rv_10.r2.dehosted_2.fastq.gz': Command '['/bin/bash', '-c', 'bowtie2 -x \'/Users/bede/Library/Application Support/hostile/human-bowtie2\' -1 \'tests/data/h37rv_10.r1.fastq.gz\' -2 \'tests/data/h37rv_10.r2.fastq.gz\' -k 1 --mm -p 10| tee >(samtools view -F 256 -c - > test/h37rv_10.r1.reads_in.txt)| samtools view --threads 5 -f 12 - | tee >(samtools view -F 256 -c - > test/h37rv_10.r1.reads_out.txt) | awk \'BEGIN{FS=OFS="\\t"} {$1=int((NR+1)/2)" "; print $0}\' | samtools fastq --threads 5 -c 6 -N -1 \'test/h37rv_10.r1.dehosted_1.fastq.gz\' -2 \'test/h37rv_10.r2.dehosted_2.fastq.gz\'']' returned non-zero exit status 1. Dehosting: 100%|████████████████████████████████████████████████████████████████████████████████████████████████████████| 1/1 [00:00<00:00, 11.09it/s] Traceback (most recent call last): File "/Users/bede/miniconda3/envs/hostile/bin/hostile", line 8, in <module> sys.exit(main()) File "/Users/bede/Research/Git/hostile/src/hostile/cli.py", line 68, in main defopt.run( File "/Users/bede/miniconda3/envs/hostile/lib/python3.10/site-packages/defopt.py", line 356, in run return call() File "/Users/bede/Research/Git/hostile/src/hostile/cli.py", line 28, in dehost stats = lib.dehost_paired_fastqs( File "/Users/bede/Research/Git/hostile/src/hostile/lib.py", line 140, in dehost_paired_fastqs stats = gather_stats(fastqs, out_dir=out_dir) File "/Users/bede/Research/Git/hostile/src/hostile/lib.py", line 89, in gather_stats n_reads_in = util.parse_count_file(n_reads_in_path) File "/Users/bede/Research/Git/hostile/src/hostile/util.py", line 55, in parse_count_file with open(path, "r") as fh: FileNotFoundError: [Errno 2] No such file or directory: 'test/h37rv_10.r1.reads_in.txt'
Fixed in b20bd87