When combining the microscope setup with the real-time triangulation-based focus sensor I figure problems with the focus data coming from the sensor is varying at different points of the multiwell plate.
I hope this is due to the bad quality of the bottom of this multiwell plate. It's an all-plastic one for cell culture/ELISA (?), so I hope a pure glass-bottom dish has fewer variations in its surface roughness.
@theosanderson @olas @jordicarr do you have any feeling about the glass surface of your imaging 96 well plates?
The numbers on the right hand side represent the focus value. Since the sensor is mounted on the objective, it should always be the same value..
When combining the microscope setup with the real-time triangulation-based focus sensor I figure problems with the focus data coming from the sensor is varying at different points of the multiwell plate.
I hope this is due to the bad quality of the bottom of this multiwell plate. It's an all-plastic one for cell culture/ELISA (?), so I hope a pure glass-bottom dish has fewer variations in its surface roughness. @theosanderson @olas @jordicarr do you have any feeling about the glass surface of your imaging 96 well plates?
The numbers on the right hand side represent the focus value. Since the sensor is mounted on the objective, it should always be the same value..