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beniroquai / TIRFImager

This is an attempt to add a TIRF Module to any microscope that can hold 96 well plates.
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it doesnt work with 80x Objective #9

Open ranranking opened 1 month ago

ranranking commented 1 month ago

I tried the 80x objective, but the working distance is quite small and also the objective itself is thick. It doesnt work with current condition.

Maybe one point to disscuss with Oystein. If we can have longer chip, longer than a microscope slide, 1.5x sized as slide, maybe we can have bigger window on the printed part in order to let the objective fit through.

do we also have something like 40x? maybe they will have a bit longer working distance? @beniroquai

beniroquai commented 1 month ago

The problem with the long chips is that the loss of intensity along the wave guide is quite severe. So you could not really do any storm at the most far away point from the coupling pulls up I think it would be nice idea to have a 40x lens. Therefore we should order some pieces from view solutions what's up your mind to have a look what's the highest na with this magnification?