Tax4fun input file post DADA2 processing

[yugi1001]

Hi,

not exactly an issue but request fro help

I am unable to get a output file which can be used by Tax4Fun post Dada2 processing. I tried the otu_table and write_biom options to get a table containing the required information and it did not work. I think this would enable a truly useful way to do all analyses in R starting from raw data to functional interpretation.

can you suggest a way forward?

thanks Yugandhar

[benjjneb]

I'm not familiar with Tax4Fun. Do they specify the input file required somewhere?

[yugi1001]

Hi Ben,

many thanks for the response. The Tax4Fun uses a biom format OTU table output from Qiime. "OTU tables were subsequently calculated using the 'make_otu_table.py' script." Text format files (Qiime output) can also be imported into Tax4Fun. Can also import SILVAngs output file in csv format.

So the issue is how to generate a biom format file from DADA2 output which is identical to either Qiime/SILVAngs output files.

Thanks again. Yugandhar

[yugi1001]

Their website is not very helpful for non-experts in metagenomic data analysis. http://tax4fun.gobics.de/

[benjjneb]

There are two completely different and incompatible versions of the biom file format. The biomformat package outputs .biom v1 files. Perhaps this needs .biom v2 (or v2.1) files? I believe QIIME now outputs in v2 or v2.1

I think there might be a utility to do that here: http://biom-format.org/documentation/biom_conversion.html

@joey711

[yugi1001]

It appears that the current biom format causes problems and Tax4Fun recommends converting it to a txt file for further analysis.

The biom-format instructions are for Qiime and I was wondering if a similar work around was available for R. I installed biom but could not figure out how to do it. I understood (belatedly) that the write_biom does not "make a biom file. Can you suggest how to "make a biom" file in R with the DADA2 output?

Thanks

On Wed, Sep 28, 2016 at 12:05 PM, benjjneb notifications@github.com wrote:

There are two completely different and incompatible versions of the biom file format. The biomformat package outputs .biom v1 files. Perhaps this needs .biom v2 (or v2.1) files? I believe QIIME now outputs in v2 or v2.1

I think there might be a utility to do that here: http://biom-format.org/ documentation/biom_conversion.html

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/benjjneb/dada2/issues/125#issuecomment-250083923, or mute the thread https://github.com/notifications/unsubscribe-auth/AKWJLar6bxkDrRCd1F2eOPseVf02BHauks5qugrLgaJpZM4KHZIu .

Yugandhar Reddy

[yugi1001]

Apologies, just realised biomformat has a make_biom option. will try that.

On Wed, Sep 28, 2016 at 12:05 PM, benjjneb notifications@github.com wrote:

There are two completely different and incompatible versions of the biom file format. The biomformat package outputs .biom v1 files. Perhaps this needs .biom v2 (or v2.1) files? I believe QIIME now outputs in v2 or v2.1

I think there might be a utility to do that here: http://biom-format.org/ documentation/biom_conversion.html

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/benjjneb/dada2/issues/125#issuecomment-250083923, or mute the thread https://github.com/notifications/unsubscribe-auth/AKWJLar6bxkDrRCd1F2eOPseVf02BHauks5qugrLgaJpZM4KHZIu .

Yugandhar Reddy

[joey711]

The "biom" package is deprecated. I should take it off of CRAN actually. The "biomformat" package on Bioconductor is legit. I believe it should be able to do what you need here.

Would help a lot if you could define which version of biom-format, and what additional specs are required.

Conversely, if Tax4Fun is an R program, it might be that no translation into an on-disk file is needed, just a method that coerces the dada2 output into the object the Tax4Fun expects to encounter.

[yugi1001]

Ben,

Tax4Fun needs biom format 1.0.0. Can you suggest a way to get either biom or its equivalent txt file please.

When I use make_biom, the file output cannot be read by read_biom and i get an error.

Thanks Yugandhar

On Thu, Sep 29, 2016 at 6:56 AM, Paul J. McMurdie notifications@github.com wrote:

The "biom" package is deprecated. I should take it off of CRAN actually. The "biomformat" package on Bioconductor is legit. I believe it should be able to do what you need here.

Would help a lot if you could define which version of biom-format, and what additional specs are required.

Conversely, if Tax4Fun is an R program, it might be that no translation into an on-disk file is needed, just a method that coerces the dada2 output into the object the Tax4Fun expects to encounter.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/benjjneb/dada2/issues/125#issuecomment-250347106, or mute the thread https://github.com/notifications/unsubscribe-auth/AKWJLZk3-eQH5Db-oSpghbxi1opvagivks5quxO_gaJpZM4KHZIu .

Yugandhar Reddy

[joey711]

@yugi1001 I don't think that's a specification that we want to support because it is an old version of the biom-format. This request is probably beyond the scope of the dada2 package. It probably makes more sense to ask the developers of Tax4Fun to support the current biom-format.

[joey711]

@yugi1001 Please also answer my question about whether Tax4Fun is an R program. If so, quibbling about the file format is pointless, because we won't need to write to disk to pass the data, just define a coercion method from dada2 data class to whatever Tax4Fun expects.

[spholmes]

yes, http://tax4fun.gobics.de/RPackage/Readme_Tax4Fun.pdf

On Fri, Sep 30, 2016 at 9:31 AM, Paul J. McMurdie notifications@github.com wrote:

@yugi1001 https://github.com/yugi1001 Please also answer my question about whether Tax4Fun is an R program. If so, quibbling about the file format is pointless, because we won't need to write to disk to pass the data, just define a coercion method from dada2 data class to whatever Tax4Fun expects.

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/benjjneb/dada2/issues/125#issuecomment-250790610, or mute the thread https://github.com/notifications/unsubscribe-auth/ABJcvQVeEzbfE652Jlzp90Zm4VcU0tURks5qvTlUgaJpZM4KHZIu .

Susan Holmes Professor, Statistics and BioX John Henry Samter Fellow in Undergraduate Education Sequoia Hall, 390 Serra Mall Stanford, CA 94305 http://www-stat.stanford.edu/~susan/

[joey711]

Great, so the next step is to figure what R class(es) are expected after their import step...

[yugi1001]

Sorry for the delay but I was unable to download the example biom/txt files which Tax4Fun can recognize use. I have attached a biom and a txt file to illustrate the format in which data needs to be presented to Tax4Fun. Hope this format can be mimicked in DADA2.

Thanks Yugandhar

On Fri, Sep 30, 2016 at 10:48 PM, Paul J. McMurdie <notifications@github.com

wrote:

Great, so the next step is to figure what R class(es) are expected after their import step...

— You are receiving this because you were mentioned. Reply to this email directly, view it on GitHub https://github.com/benjjneb/dada2/issues/125#issuecomment-250801790, or mute the thread https://github.com/notifications/unsubscribe-auth/AKWJLUk9Blz2mXEJRZqKRoy3U7bpIVcGks5qvUR6gaJpZM4KHZIu .

Yugandhar Reddy

Constructed from biom file

OTU ID GNmat.10.22mm GNmat.1.2mm GNmat.6.10mm GNmat.22.34mm GNmat.34.49mm GNmat.2.3mm GNmat.4.5mm GNmat.3.4mm GNmat.0.1mm GNmat.5.6mm taxonomy

denovo0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; ST-12K33; uncultured organism denovo1 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Haliea; uncultured organism denovo2 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo3 14.0 1.0 12.0 23.0 20.0 4.0 1.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Pla1 lineage; uncultured organism denovo4 7.0 0.0 0.0 3.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo5 5.0 5.0 8.0 4.0 4.0 6.0 2.0 8.0 1.0 1.0 Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Desulfomonile; uncultured organism denovo6 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo7 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Cytophagia; Order II Incertae Sedis; Rhodothermaceae; Salinibacter; uncultured organism denovo8 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Verrucomicrobia; Spartobacteria; Chthoniobacterales; 01D2Z36; uncultured organism denovo9 7.0 0.0 24.0 9.0 0.0 0.0 6.0 0.0 2.0 8.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; 08D2Z94 hypersaline microbial mat group; uncultured organism denovo10 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo11 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Hyphomonadaceae; Ponticaulis; uncultured organism denovo12 0.0 1.0 0.0 0.0 0.0 1.0 0.0 2.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo13 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division OD1; uncultured bacterium denovo14 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo15 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Hyphomicrobiaceae; Dichotomicrobium; uncultured organism denovo16 0.0 0.0 1.0 0.0 1.0 0.0 1.0 2.0 1.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; 062DZ93 hypersaline microbial mat group; uncultured organism denovo17 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Porphyrobacter; uncultured organism denovo18 0.0 1.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo19 0.0 0.0 0.0 0.0 0.0 1.0 0.0 1.0 0.0 0.0 Bacteria; Candidate division SR1; uncultured organism denovo20 0.0 1.0 0.0 1.0 1.0 0.0 5.0 19.0 4.0 6.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; uncultured; uncultured organism denovo21 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; uncultured organism denovo22 4.0 2.0 7.0 1.0 0.0 5.0 3.0 2.0 1.0 2.0 Bacteria; Bacteroidetes; SB-1; uncultured organism denovo23 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo24 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; 09D2Z46; uncultured organism denovo25 0.0 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; uncultured; uncultured organism denovo26 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo27 1.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; GR-WP33-58; uncultured organism denovo28 0.0 0.0 3.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo29 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Armatimonadetes; uncultured organism denovo30 1.0 0.0 0.0 4.0 5.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo31 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo32 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; SM1A07; uncultured organism denovo33 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio; uncultured organism denovo34 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo35 0.0 11.0 0.0 0.0 2.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Leptospiraceae; uncultured; uncultured organism denovo36 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Thalassospira; uncultured organism denovo37 0.0 1.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo38 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo39 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; SM1A07; uncultured organism denovo40 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Verrucomicrobia; OPB35 soil group; uncultured organism denovo41 0.0 2.0 1.0 0.0 1.0 2.0 3.0 1.0 0.0 2.0 Bacteria; Proteobacteria; Deltaproteobacteria; 43F-1404R; uncultured organism denovo42 3.0 6.0 0.0 0.0 0.0 6.0 3.0 8.0 7.0 2.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; Puniceicoccus; uncultured organism denovo43 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; uncultured organism denovo44 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; MAT-CR-H6-H10; uncultured organism denovo45 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Marinilabiaceae; uncultured; uncultured organism denovo46 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo47 0.0 1.0 0.0 0.0 0.0 1.0 0.0 1.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; SM1A02; uncultured organism denovo48 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo49 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Hyphomonadaceae; uncultured; uncultured organism denovo50 2.0 0.0 8.0 3.0 1.0 4.0 6.0 5.0 1.0 1.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo51 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; 08D2Z94 hypersaline microbial mat group; uncultured organism denovo52 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo53 1.0 0.0 0.0 1.0 3.0 0.0 4.0 1.0 0.0 4.0 Bacteria; Candidate division BRC1; uncultured organism denovo54 1.0 2.0 0.0 2.0 9.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo55 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Candidate division BRC1; uncultured organism denovo56 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; MD2896-B258; uncultured organism denovo57 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo58 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Armatimonadetes; uncultured organism denovo59 0.0 0.0 1.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo60 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; OM190; uncultured bacterium denovo61 1.0 5.0 3.0 0.0 0.0 2.0 1.0 3.0 0.0 2.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo62 1.0 0.0 0.0 0.0 0.0 0.0 1.0 1.0 0.0 1.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; MAT-CR-H6-H10; uncultured organism denovo63 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; Marinoscillum; uncultured organism denovo64 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Verrucomicrobia; Opitutae; vadinHA64; uncultured organism denovo65 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 2.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo66 0.0 4.0 0.0 0.0 0.0 7.0 0.0 0.0 1.0 0.0 Bacteria; Thermotogae; Thermotogae; Thermotogales; Thermotogaceae; SC103; uncultured organism denovo67 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 3.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Tistlia; uncultured organism denovo68 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo69 2.0 2.0 5.0 1.0 0.0 2.0 1.0 2.0 2.0 1.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; uncultured; uncultured organism denovo70 0.0 0.0 0.0 0.0 1.0 11.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; Class Incertae Sedis; Order Incertae Sedis; Family Incertae Sedis; Prolixibacter; uncultured organism denovo71 0.0 1.0 0.0 0.0 0.0 4.0 0.0 1.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae; Desulfitibacter; uncultured organism denovo72 12.0 0.0 6.0 7.0 4.0 4.0 11.0 4.0 2.0 3.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; SBXY-748; uncultured organism denovo73 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; Victivallis; uncultured organism denovo74 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo75 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo76 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; HOC36; uncultured organism denovo77 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; NPL-UPA2; uncultured organism denovo78 4.0 1.0 3.0 1.0 7.0 1.0 2.0 0.0 1.0 1.0 Bacteria; Gemmatimonadetes; Gemmatimonadetes; BD2-11 terrestrial group; uncultured organism denovo79 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Pla1 lineage; uncultured organism denovo80 1.0 0.0 0.0 2.0 3.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo81 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Euhalothece; uncultured organism denovo82 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo83 0.0 0.0 1.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium; uncultured organism denovo84 0.0 1.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; BD1-5; uncultured organism denovo85 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo86 0.0 0.0 1.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo87 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; E6aD10; uncultured organism denovo88 0.0 2.0 1.0 1.0 0.0 4.0 3.0 7.0 2.0 4.0 Bacteria; SBYG-2791; uncultured organism denovo89 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo90 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 3.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Chitinophagaceae; uncultured; uncultured organism denovo91 0.0 0.0 0.0 1.0 0.0 0.0 0.0 3.0 0.0 2.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo92 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; SAR406 clade(Marine group A); uncultured organism denovo93 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured bacterium denovo94 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 1.0 Bacteria; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; Rapidithrix; uncultured organism denovo95 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Bacteroidetes; SBXY-6786; uncultured organism denovo96 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; OM182 clade; uncultured organism denovo97 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; SM1A02; uncultured organism denovo98 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Hyphomicrobiaceae; Dichotomicrobium; uncultured organism denovo99 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; uncultured; uncultured organism denovo100 0.0 0.0 0.0 2.0 2.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; 09D2Z46; uncultured organism denovo101 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Euhalothece; uncultured organism denovo102 0.0 0.0 2.0 1.0 3.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; 09D2Z46; uncultured organism denovo103 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Hyphomicrobiaceae; Dichotomicrobium; uncultured organism denovo104 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo105 0.0 1.0 1.0 0.0 0.0 1.0 1.0 4.0 0.0 4.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; Phycisphaera; uncultured organism denovo106 4.0 1.0 19.0 1.0 0.0 8.0 9.0 10.0 0.0 15.0 Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales; Cryomorphaceae; NS7 marine group; uncultured organism denovo107 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo108 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; NPL-UPA2; uncultured organism denovo109 0.0 0.0 1.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo110 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo111 1.0 0.0 0.0 3.0 12.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo112 0.0 1.0 0.0 0.0 0.0 5.0 0.0 0.0 0.0 2.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo113 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo114 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; GR-WP33-30; uncultured organism denovo115 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; 09D2Z48; uncultured organism denovo116 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo117 1.0 0.0 0.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; WCHB1-41; uncultured organism denovo118 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 1.0 0.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo119 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Marinilabiaceae; uncultured; uncultured organism denovo120 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; uncultured; uncultured organism denovo121 1.0 1.0 0.0 0.0 0.0 1.0 0.0 1.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo122 1.0 5.0 1.0 0.0 0.0 2.0 8.0 4.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; WCHB1-41; uncultured organism denovo123 10.0 0.0 22.0 1.0 0.0 4.0 56.0 11.0 2.0 30.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo124 0.0 0.0 1.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; 4C0d-2; uncultured organism denovo125 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo126 0.0 2.0 0.0 0.0 1.0 1.0 2.0 0.0 14.0 1.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Euhalothece; uncultured organism denovo127 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Chitinophagaceae; uncultured; uncultured organism denovo128 3.0 0.0 0.0 8.0 5.0 0.0 2.0 1.0 2.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo129 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; OM182 clade; uncultured organism denovo130 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Candidate division OP3; uncultured organism denovo131 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Chlamydiae; Chlamydiae; Chlamydiales; Chlamydiaceae; uncultured; uncultured organism denovo132 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Chloroflexi; Caldilineae; Caldilineales; Caldilineaceae; uncultured; uncultured organism denovo133 0.0 5.0 0.0 0.0 1.0 2.0 0.0 0.0 2.0 0.0 Bacteria; Bacteroidetes; SM1A07; uncultured organism denovo134 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales; Flavobacteriaceae; Croceitalea; uncultured Bacteroidetes bacterium denovo135 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; GR-WP33-58; uncultured organism denovo136 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Phormidium; uncultured organism denovo137 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; P. palm C-A 51; uncultured organism denovo138 0.0 0.0 0.0 0.0 0.0 1.0 0.0 3.0 0.0 2.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; Puniceicoccus; uncultured organism denovo139 0.0 0.0 0.0 0.0 0.0 1.0 2.0 3.0 1.0 1.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; Pirellula; uncultured organism denovo140 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo141 0.0 0.0 0.0 4.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division WS3; uncultured organism denovo142 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Candidate division OD1; marine metagenome denovo143 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 4.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo144 0.0 0.0 1.0 0.0 0.0 5.0 4.0 5.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo145 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; SM1A02; uncultured organism denovo146 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Pla3 lineage; uncultured organism denovo147 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo148 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Marinilabiaceae; uncultured; uncultured organism denovo149 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Pla4 lineage; uncultured organism denovo150 3.0 0.0 0.0 5.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Hyphomicrobiaceae; Dichotomicrobium; uncultured alpha proteobacterium denovo151 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; E6aC02; uncultured organism denovo152 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfobacterium; uncultured organism denovo153 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Bdellovibrionales; Bacteriovoracaceae; uncultured; uncultured organism denovo154 3.0 4.0 4.0 1.0 0.0 1.0 0.0 1.0 9.0 5.0 Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Hyphomonadaceae; Hyphomonas; uncultured organism denovo155 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; 0319-6G20; uncultured organism denovo156 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo157 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; SAR406 clade(Marine group A); uncultured organism denovo158 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; BD1-5; uncultured organism denovo159 0.0 6.0 0.0 0.0 1.0 3.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo160 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; Incertae Sedis; uncultured organism denovo161 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo162 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Desulfomonile; uncultured organism denovo163 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo164 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 1.0 0.0 Bacteria; Verrucomicrobia; Spartobacteria; Chthoniobacterales; Xiphinematobacteraceae; Candidatus Xiphinematobacter; uncultured organism denovo165 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Roseivivax; Roseivivax sp. NT N51 denovo166 0.0 1.0 0.0 0.0 6.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; 4C0d-2; uncultured organism denovo167 0.0 4.0 0.0 0.0 2.0 3.0 5.0 3.0 8.0 3.0 Bacteria; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; Porifericola; uncultured organism denovo168 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo169 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo170 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; SM1A07; uncultured organism denovo171 0.0 0.0 1.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division JS1; uncultured organism denovo172 3.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo173 1.0 1.0 1.0 1.0 0.0 1.0 2.0 1.0 12.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo174 0.0 3.0 1.0 0.0 3.0 5.0 2.0 1.0 3.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; Lewinella; uncultured organism denovo175 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo176 0.0 0.0 0.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo177 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo178 0.0 11.0 3.0 1.0 0.0 14.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; GR-WP33-58; uncultured organism denovo179 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo180 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; 0319-6G20; uncultured organism denovo181 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Chitinophagaceae; uncultured; uncultured organism denovo182 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; uncultured organism denovo183 0.0 0.0 1.0 0.0 0.0 1.0 2.0 1.0 0.0 2.0 Bacteria; Candidate division WS3; uncultured organism denovo184 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Eggerthella; uncultured organism denovo185 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo186 1.0 0.0 0.0 0.0 1.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; OCS116 clade; uncultured organism denovo187 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 2.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; ML635J-40 aquatic group; uncultured organism denovo188 0.0 11.0 0.0 0.0 1.0 16.0 1.0 33.0 16.0 3.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo189 0.0 1.0 0.0 0.0 5.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Acidobacteria; Acidobacteria; Subgroup 3; PAUC26f; uncultured organism denovo190 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; Saprospira; uncultured organism denovo191 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo192 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; uncultured; uncultured organism denovo193 0.0 0.0 0.0 1.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Pla1 lineage; uncultured organism denovo194 1.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; BD1-5; uncultured organism denovo195 0.0 12.0 0.0 0.0 1.0 18.0 1.0 1.0 0.0 2.0 Bacteria; TA06; uncultured organism denovo196 0.0 2.0 0.0 0.0 0.0 1.0 0.0 4.0 4.0 2.0 Bacteria; Candidate division BRC1; uncultured organism denovo197 0.0 0.0 1.0 0.0 3.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo198 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo199 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo200 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo201 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division OD1; uncultured bacterium denovo202 1.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Candidate division OP3; uncultured organism denovo203 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; uncultured; uncultured organism denovo204 3.0 2.0 2.0 5.0 15.0 1.0 0.0 2.0 1.0 2.0 Bacteria; Proteobacteria; Deltaproteobacteria; FW113; uncultured organism denovo205 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; TA06; uncultured organism denovo206 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo207 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Chitinophagaceae; uncultured; uncultured organism denovo208 0.0 0.0 3.0 0.0 0.0 1.0 2.0 2.0 0.0 5.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo209 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; Pir4 lineage; uncultured organism denovo210 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; SHA-43; uncultured organism denovo211 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured Bacteroidetes bacterium denovo212 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo213 49.0 2.0 43.0 31.0 2.0 9.0 78.0 11.0 6.0 42.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; 08D2Z94 hypersaline microbial mat group; uncultured organism denovo214 10.0 4.0 37.0 27.0 2.0 7.0 26.0 19.0 1.0 12.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo215 3.0 1.0 5.0 0.0 1.0 3.0 0.0 2.0 0.0 4.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo216 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo217 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo218 4.0 0.0 5.0 4.0 6.0 1.0 0.0 1.0 0.0 5.0 Bacteria; Planctomycetes; Phycisphaerae; SHA-43; uncultured organism denovo219 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridiisalibacter; uncultured organism denovo220 13.0 0.0 18.0 5.0 11.0 0.0 1.0 0.0 1.0 0.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo221 7.0 1.0 6.0 13.0 11.0 3.0 7.0 1.0 0.0 4.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfosalsimonas; uncultured organism denovo222 1.0 0.0 0.0 0.0 0.0 1.0 3.0 0.0 0.0 1.0 Bacteria; Lentisphaerae; Lentisphaeria; B01R017; uncultured organism denovo223 0.0 0.0 0.0 0.0 0.0 1.0 0.0 1.0 0.0 0.0 Bacteria; Cyanobacteria; MLE1-12; uncultured organism denovo224 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; DB1-14; uncultured organism denovo225 0.0 2.0 3.0 0.0 0.0 0.0 4.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo226 107.0 7.0 84.0 157.0 80.0 7.0 37.0 6.0 6.0 32.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo227 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; uncultured; uncultured organism denovo228 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo229 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo230 0.0 4.0 1.0 0.0 0.0 1.0 0.0 5.0 1.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; uncultured; uncultured spirochete denovo231 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo232 0.0 5.0 1.0 0.0 1.0 9.0 1.0 8.0 0.0 6.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo233 2.0 0.0 1.0 0.0 1.0 1.0 0.0 0.0 0.0 1.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo234 0.0 1.0 0.0 0.0 0.0 0.0 0.0 1.0 1.0 1.0 Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; BD1-7 clade; uncultured organism denovo235 5.0 2.0 14.0 1.0 1.0 1.0 4.0 19.0 1.0 14.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Marinilabiaceae; uncultured; uncultured organism denovo236 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; 08D2Z94 hypersaline microbial mat group; uncultured organism denovo237 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; uncultured organism denovo238 0.0 1.0 2.0 1.0 0.0 9.0 3.0 13.0 1.0 5.0 Bacteria; BD1-5; uncultured organism denovo239 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Order Incertae Sedis; Family Incertae Sedis; Marinicella; uncultured organism denovo240 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Candidate division OD1; uncultured organism denovo241 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Euhalothece; Euhalothece sp. 'MPI 96N304' denovo242 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; uncultured; uncultured organism denovo243 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo244 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Bdellovibrionales; Bacteriovoracaceae; Peredibacter; uncultured organism denovo245 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Candidate division BRC1; uncultured organism denovo246 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum; uncultured organism denovo247 0.0 33.0 0.0 3.0 2.0 11.0 0.0 1.0 22.0 1.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo248 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo249 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Nesiotobacter; Nesiotobacter sp. H02E denovo250 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo251 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 No blast hit denovo252 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; uncultured; uncultured organism denovo253 1.0 1.0 11.0 4.0 2.0 1.0 8.0 0.0 1.0 4.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo254 0.0 0.0 1.0 0.0 0.0 0.0 2.0 1.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; SBYG-2613; uncultured organism denovo255 0.0 0.0 1.0 2.0 7.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo256 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; Pir4 lineage; uncultured organism denovo257 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Chlamydiae; Chlamydiae; Chlamydiales; Simkaniaceae; uncultured; uncultured Chlamydia sp. denovo258 0.0 1.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Acidobacteria; Subgroup 22; uncultured organism denovo259 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; MSBL9; uncultured organism denovo260 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo261 0.0 2.0 0.0 0.0 1.0 7.0 0.0 7.0 2.0 3.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo262 0.0 0.0 1.0 0.0 0.0 0.0 2.0 0.0 0.0 3.0 Bacteria; Chlorobi; Chlorobia; Chlorobiales; OPB56; uncultured organism denovo263 0.0 1.0 3.0 1.0 0.0 0.0 2.0 2.0 1.0 2.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo264 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; uncultured; uncultured organism denovo265 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 4.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rubribacterium; uncultured organism denovo266 0.0 0.0 0.0 0.0 4.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophobacterales; Syntrophobacteraceae; uncultured; uncultured organism denovo267 0.0 0.0 1.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo268 0.0 0.0 1.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; 0319-6G20; uncultured organism denovo269 2.0 2.0 0.0 1.0 0.0 0.0 1.0 1.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridiisalibacter; uncultured organism denovo270 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; Phaselicystidaceae; Phaselicystis; uncultured organism denovo271 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; WCHB1-41; uncultured organism denovo272 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; Pir4 lineage; uncultured organism denovo273 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Roseibacterium; uncultured organism denovo274 0.0 2.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo275 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo276 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 3.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo277 0.0 2.0 0.0 1.0 0.0 1.0 1.0 3.0 1.0 1.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo278 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; uncultured; uncultured organism denovo279 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo280 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Sediminimonas; uncultured organism denovo281 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division OP8; uncultured organism denovo282 1.0 2.0 0.0 0.0 0.0 0.0 2.0 2.0 0.0 6.0 Bacteria; Verrucomicrobia; Spartobacteria; Chthoniobacterales; 01D2Z36; uncultured organism denovo283 11.0 2.0 4.0 4.0 3.0 0.0 2.0 4.0 6.0 3.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Roseibacterium; uncultured organism denovo284 0.0 0.0 0.0 1.0 1.0 0.0 0.0 0.0 5.0 0.0 Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Family Incertae Sedis; Candidatus Chlorothrix; uncultured organism denovo285 2.0 0.0 2.0 1.0 1.0 7.0 6.0 7.0 0.0 1.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo286 9.0 1.0 4.0 6.0 5.0 1.0 7.0 13.0 0.0 6.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; uncultured; uncultured organism denovo287 0.0 2.0 0.0 0.0 0.0 5.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo288 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo289 0.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 1.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Order Incertae Sedis; Family Incertae Sedis; Marinicella; uncultured organism denovo290 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; uncultured; uncultured organism denovo291 0.0 3.0 0.0 0.0 2.0 1.0 0.0 0.0 23.0 1.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Spirulina; uncultured organism denovo292 0.0 8.0 0.0 0.0 0.0 18.0 0.0 2.0 1.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo293 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; WCHB1-41; uncultured organism denovo294 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 7.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo295 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo296 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Candidate division OP3; uncultured organism denovo297 1.0 1.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; Planctomyces; uncultured organism denovo298 0.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; TM6; uncultured organism denovo299 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo300 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Candidate division OP3; uncultured organism denovo301 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 7.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; uncultured bacterium denovo302 1.0 2.0 0.0 9.0 9.0 0.0 2.0 1.0 5.0 1.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo303 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Brevinemataceae; Brevinema; uncultured organism denovo304 0.0 1.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfovibrionales; Desulfohalobiaceae; Desulfovermiculus; uncultured organism denovo305 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; MSBL9; uncultured organism denovo306 4.0 0.0 4.0 6.0 12.0 0.0 1.0 0.0 0.0 2.0 Bacteria; Planctomycetes; Phycisphaerae; 062DZ93 hypersaline microbial mat group; uncultured organism denovo307 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo308 1.0 0.0 0.0 2.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo309 6.0 0.0 6.0 3.0 2.0 3.0 4.0 1.0 1.0 2.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo310 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo311 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo312 14.0 1.0 5.0 4.0 16.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo313 0.0 16.0 0.0 0.0 1.0 25.0 0.0 2.0 2.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Family XII Incertae Sedis; Fusibacter; uncultured organism denovo314 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Verrucomicrobia; Class Incertae Sedis; Order Incertae Sedis; Family Incertae Sedis; Candidatus Methylacidiphilum; uncultured organism denovo315 0.0 0.0 0.0 0.0 5.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo316 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chlorobi; Ignavibacteria; Ignavibacteriales; Ignavibacteriaceae; Ignavibacterium; uncultured organism denovo317 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo318 0.0 6.0 0.0 0.0 0.0 2.0 1.0 0.0 1.0 1.0 Bacteria; Candidate division SR1; uncultured organism denovo319 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo320 0.0 28.0 0.0 0.0 4.0 14.0 1.0 7.0 1.0 7.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo321 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo322 1.0 0.0 1.0 1.0 0.0 0.0 2.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; Incertae Sedis; uncultured organism denovo323 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 Bacteria; Candidate division WS6; uncultured organism denovo324 0.0 7.0 1.0 1.0 2.0 4.0 1.0 0.0 30.0 3.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo325 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; Sandaracinaceae; Sandaracinus; uncultured organism denovo326 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; Lewinella; uncultured organism denovo327 4.0 1.0 1.0 7.0 3.0 0.0 1.0 0.0 0.0 1.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; uncultured; uncultured organism denovo328 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; Incertae Sedis; uncultured organism denovo329 43.0 6.0 34.0 6.0 0.0 5.0 26.0 19.0 3.0 27.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; WCHB1-69; uncultured organism denovo330 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Brachyspiraceae; Brachyspira; uncultured organism denovo331 0.0 0.0 1.0 0.0 0.0 0.0 2.0 0.0 0.0 1.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo332 0.0 0.0 0.0 1.0 1.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo333 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; 062DZ93 hypersaline microbial mat group; uncultured organism denovo334 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; MAT-CR-H6-H10; uncultured organism denovo335 2.0 0.0 1.0 10.0 15.0 2.0 3.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo336 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo337 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; SEEP-SRB1; uncultured organism denovo338 0.0 0.0 0.0 0.0 0.0 4.0 0.0 0.0 0.0 0.0 Bacteria; Chlorobi; Chlorobia; Chlorobiales; OPB56; uncultured organism denovo339 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; Incertae Sedis; uncultured organism denovo340 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chlorobi; Chlorobia; Chlorobiales; OPB56; uncultured organism denovo341 3.0 1.0 0.0 0.0 1.0 2.0 6.0 5.0 2.0 4.0 Bacteria; Planctomycetes; vadinHA49; uncultured organism denovo342 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; uncultured; uncultured organism denovo343 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Brachyspiraceae; Brachyspira; uncultured organism denovo344 0.0 89.0 10.0 3.0 7.0 164.0 0.0 6.0 2.0 6.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; E6aC02; uncultured organism denovo345 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales; Cryomorphaceae; Cryomorpha; uncultured organism denovo346 0.0 1.0 0.0 0.0 0.0 2.0 0.0 1.0 1.0 0.0 Bacteria; Armatimonadetes; uncultured organism denovo347 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; Phycisphaera; uncultured organism denovo348 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo349 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Chitinophagaceae; uncultured; uncultured organism denovo350 0.0 0.0 1.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; uncultured; uncultured organism denovo351 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Marinilabiaceae; uncultured; uncultured organism denovo352 2.0 0.0 0.0 0.0 0.0 3.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Tropicimonas; uncultured organism denovo353 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo354 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo355 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Haliea; uncultured organism denovo356 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo357 0.0 33.0 5.0 1.0 2.0 83.0 2.0 62.0 35.0 4.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; ML635J-40 aquatic group; uncultured organism denovo358 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; uncultured; uncultured organism denovo359 1.0 0.0 5.0 0.0 0.0 1.0 1.0 3.0 0.0 1.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo360 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Leptolyngbya; uncultured organism denovo361 0.0 30.0 2.0 2.0 4.0 22.0 4.0 32.0 25.0 5.0 Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales; Cryomorphaceae; Cryomorpha; uncultured organism denovo362 2.0 1.0 1.0 0.0 0.0 2.0 11.0 3.0 1.0 10.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo363 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Candidate division WS6; uncultured organism denovo364 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo365 0.0 2.0 0.0 0.0 3.0 0.0 1.0 0.0 8.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; NKB5; uncultured organism denovo366 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo367 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo368 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; NPL-UPA2; uncultured organism denovo369 1.0 0.0 0.0 1.0 0.0 0.0 0.0 1.0 0.0 1.0 Bacteria; Candidate division BRC1; uncultured organism denovo370 5.0 0.0 1.0 3.0 4.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae; uncultured; uncultured organism denovo371 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; MAT-CR-H6-H10; uncultured organism denovo372 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo373 0.0 0.0 2.0 1.0 0.0 0.0 0.0 0.0 0.0 2.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; 08D2Z94 hypersaline microbial mat group; uncultured organism denovo374 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; MgMjR-022; uncultured organism denovo375 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo376 0.0 0.0 1.0 0.0 2.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; R76-B128; uncultured organism denovo377 1.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured spirochete denovo378 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; uncultured; uncultured organism denovo379 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo380 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo381 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Sh765B-TzT-29; uncultured organism denovo382 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; GR-WP33-58; uncultured organism denovo383 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; SAR324 clade(Marine group B); uncultured organism denovo384 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Candidate division BRC1; uncultured organism denovo385 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; uncultured; uncultured organism denovo386 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 1.0 Bacteria; BD1-5; uncultured organism denovo387 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo388 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; WCHB1-69; uncultured organism denovo389 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo390 2.0 1.0 2.0 2.0 0.0 3.0 2.0 1.0 3.0 6.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo391 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Bacteroidetes; SB-5; uncultured organism denovo392 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division BRC1; uncultured organism denovo393 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo394 0.0 2.0 0.0 0.0 0.0 2.0 1.0 6.0 0.0 2.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo395 2.0 0.0 5.0 17.0 8.0 2.0 2.0 0.0 1.0 1.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; uncultured; uncultured organism denovo396 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; TM6; uncultured bacterium denovo397 5.0 0.0 2.0 1.0 1.0 1.0 5.0 0.0 0.0 0.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo398 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Wenxinia; uncultured organism denovo399 0.0 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; E6aC02; uncultured organism denovo400 0.0 0.0 0.0 0.0 0.0 3.0 0.0 0.0 0.0 2.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo401 0.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division OP8; uncultured organism denovo402 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Thermotogae; Thermotogae; Thermotogales; Thermotogaceae; SC103; uncultured organism denovo403 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Proteobacteria; Deltaproteobacteria; SAR324 clade(Marine group B); uncultured organism denovo404 1.0 0.0 0.0 3.0 4.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; mle1-8; uncultured organism denovo405 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Family XII Incertae Sedis; Fusibacter; uncultured organism denovo406 0.0 0.0 0.0 3.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Marinilabiaceae; uncultured; uncultured organism denovo407 0.0 1.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Spirochaetaceae; Spirochaeta; uncultured organism denovo408 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 1.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; marine group; uncultured organism denovo409 1.0 0.0 0.0 10.0 15.0 1.0 1.0 0.0 1.0 1.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; AKAU3564 sediment group; uncultured organism denovo410 0.0 0.0 3.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Candidate division BRC1; uncultured organism denovo411 0.0 1.0 5.0 0.0 0.0 0.0 2.0 0.0 0.0 0.0 Bacteria; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; Roseibacillus; uncultured organism denovo412 0.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo413 0.0 6.0 0.0 0.0 1.0 0.0 0.0 5.0 0.0 0.0 Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Methylohalomonas; uncultured organism denovo414 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo415 0.0 3.0 4.0 0.0 0.0 1.0 0.0 0.0 3.0 2.0 Bacteria; Planctomycetes; Planctomycetacia; Planctomycetales; Planctomycetaceae; Blastopirellula; uncultured organism denovo416 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; uncultured; uncultured organism denovo417 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Bacteroidetes; BD2-2; uncultured organism denovo418 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Fibrobacteres; Fibrobacteria; Fibrobacterales; MAT-CR-H6-H10; uncultured organism denovo419 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo420 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Microcoleus; uncultured organism denovo421 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; Puniceicoccus; uncultured organism denovo422 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 14.0 1.0 Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Thiothrix; uncultured organism denovo423 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Planctomycetes; Phycisphaerae; SHA-43; uncultured organism denovo424 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; DB1-14; uncultured organism denovo425 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 10.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; GR-WP33-58; uncultured organism denovo426 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Candidate division BRC1; uncultured organism denovo427 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Verrucomicrobia; Opitutae; Puniceicoccales; Puniceicoccaceae; Cerasicoccus; uncultured organism denovo428 0.0 0.0 0.0 0.0 2.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; uncultured; uncultured organism denovo429 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo430 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 1.0 1.0 Bacteria; Candidate division OD1; uncultured organism denovo431 8.0 0.0 0.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Lentisphaerae; Lentisphaeria; WCHB1-41; uncultured organism denovo432 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo433 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 No blast hit denovo434 1.0 0.0 1.0 17.0 3.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Caldilineae; Caldilineales; Caldilineaceae; uncultured; uncultured organism denovo435 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; uncultured; uncultured organism denovo436 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Spirochaetae; Spirochaetes; Spirochaetales; Brachyspiraceae; Brachyspira; uncultured organism denovo437 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridiisalibacter; uncultured organism denovo438 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; Phycisphaera; uncultured organism denovo439 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; uncultured; uncultured organism denovo440 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo441 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; 43F-1404R; uncultured organism denovo442 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales; GR-WP33-58; uncultured organism denovo443 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0 Bacteria; Actinobacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis; uncultured bacterium denovo444 0.0 0.0 0.0 0.0 0.0 0.0 1.0 1.0 0.0 0.0 Bacteria; Cyanobacteria; ML635J-21; uncultured organism denovo445 0.0 1.0 1.0 0.0 0.0 0.0 0.0 1.0 1.0 1.0 Bacteria; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Saprospiraceae; Lewinella; uncultured organism denovo446 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 Bacteria; Cyanobacteria; 4C0d-2; uncultured organism denovo447 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 Bacteria; Cyanobacteria; Cyanobacteria; SubsectionIII; FamilyI; Oscillatoria; uncultured organism denovo448 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 Bacteria; Verrucomicrobia; Spartobacteria; Chthoniobacterales; 01D2Z36; uncultured organism denovo449 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Trueperaceae; Truepera; uncultured organism denovo450 4.0 0.0 0.0 4.0 0.0 1.0 1.0 2.0 0.0 3.0 Bacteria; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; uncultured; uncultured organism denovo451 0.0 0.0 0.0 0.0 0.0 0.0 1.0 0.0 0.0 0.0 Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; 0319-6G20; uncultured organism denovo452 0.0 0.0 0.0 0.0 0.0 2.0 3.0 0.0 0.0 1.0 Bacteria; Deferribacteres; Deferribacteres; Deferribacterales; Family Incertae Sedis; Caldithrix; uncultured organism denovo453 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 1.0 Bacteria; Lentisphaerae; Lentisphaeria; Victivallales; Victivallaceae; uncultured; uncultured organism denovo454 0.0 0.0

[alexa1204]

Hi Yugandhar, That´s not a dada2 issue at all, I have the same issues using a biom file constructed by any other pipeline such as mothur or qiime - Tax4Fun just won´t read it. The fastest way is to read in the converted .txt file. However, you need to change it manually a bit according to their example file (http://tax4fun.gobics.de/ -->QIIME output: Human microbiome (HMP): download (.biom, 4.9 MB)/download (.txt, 8.5 MB)). Remove the header # Constructed from biom file, then add the 7th "uncultured organism" to each taxonomy assignment (Tax4Fun needs 7 tax levels).

I use the following for Tax4Fun: library(Tax4Fun) folderReferenceData<- "c:/Users/Alex/Desktop/SILVA123/" file<-importQIIMEData ("biom.txt") file_collapsed<-Tax4Fun(file,folderReferenceData,fctProfiling = FALSE, refProfile = "UProC",shortReadMode = TRUE,normCopyNo = TRUE) Tax4FunProfile_c<- file_collapsed$Tax4FunProfile Tax4FunProfile_c <-data.frame(t(file_collapsed$Tax4FunProfile)) write.table(Tax4FunProfile_c,"Tax4fun_collapsed.csv",sep=";")

I´m sorry, I could´nt figure out a faster way. If you are still struggling, you can also send me your biom file and I convert it for you. Hope that helps! Alex

[jgrembi]

We were able to get around this without having to convert to a text file.

As input, Tax4Fun wants a list with the sample names and then the OTU table. You can manually make that list with the dada2 output as the OTU table.
In our case, we made the dada2 output into a phyloseq object.

Tax4Fun also needs the row.names of the OTU table to be the taxonomy that will match the Silva database (need to match the format in SilvaID.RData from the SILVA123 folder that you get with Tax4Fun). So, we replaced the rownames of the OTU table with the complete taxonomy, and then used the following line of code:

list_tax4Fun <- list(sampleNames = as.character(sample_names(data)), otuTable = as(data@otu_table, "matrix"))

NOTE: On our first try, we got an error because Tax4Fun wants Silva specific taxonomy. If you used the awesome feature allowMultiple=TRUE in the dada2 assignSpecies function, you need to go back and pick one to use moving forward in Tax4Fun:(

Cheers, Jess