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benjjneb / dada2

Accurate sample inference from amplicon data with single nucleotide resolution
http://benjjneb.github.io/dada2/
GNU Lesser General Public License v3.0
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16S/18S/ITS #1647

Closed agnieszka6552 closed 4 months ago

agnieszka6552 commented 1 year ago

I have a data from 16S/18S/ITS Amplicon Sequencing. Can I use dada2 for analyzing such data? Should I divide them into 16S, 18S and ITS subsets as the reads have different lengths?

benjjneb commented 1 year ago

Yes it can. If you have a way to divide things out before DADA2 that's ideal. If you don't, DADA2 will still work, but you'll probably have to be more delibrate about how you do some downstream steps like taxonomic ID.