Closed splaisan closed 5 years ago
I tried the following:
bin/bg7 -n bg7_example \ -g bg7_example_input_files/escherichia_coli_o104_h4_str._ty-2482_1_supercontigs.fasta \ -p bg7_example_input_files/EHEC_ReferenceProteins_17_08_2012.fasta \ -r bg7_example_input_files/EHEC_Reference_RNAs.frn \ -c bg7_example_input_files/genetic_code.txt \ -o bg7_example \ -G bg7_example_input_files/GenBankExternalData.xml
logging your params to /opt/biotools/BG7/bg7_example/params.log
it looks like this machine has 88 cores, will use that for blast settings
creating RNAs blast db
RNA blast db created: /opt/biotools/BG7/bg7_example/bg7_example.rnas.db
if you want, you can check makeblastdb log: /opt/biotools/BG7/bg7_example/bg7_example.rnas.db.log
running blastn: RNAs vs genome sequence
done! will do a basic results check now
checking if blast xml output file looks ok...
$ ll bg7_example/
total 128K
drwxr-xr-x 2 u0002316 domain users 4.0K Aug 14 11:30 .
drwxr-xr-x 9 u0002316 domain users 4.0K Aug 14 11:29 ..
-rw-r--r-- 1 u0002316 domain users 69K Aug 14 11:30 bg7_example_RNA_blastn.xml
-rw-r--r-- 1 u0002316 domain users 361 Aug 14 11:30 bg7_example.rnas.db.log
-rw-r--r-- 1 u0002316 domain users 22K Aug 14 11:30 bg7_example.rnas.db.nhr
-rw-r--r-- 1 u0002316 domain users 2.0K Aug 14 11:30 bg7_example.rnas.db.nin
-rw-r--r-- 1 u0002316 domain users 12K Aug 14 11:30 bg7_example.rnas.db.nsq
-rw-r--r-- 1 u0002316 domain users 529 Aug 14 11:30 params.log
this does not look like completed to me ...
You can always try Prokka https://github.com/tseemann/prokka :-)
Thanks @tseemann; I was hoping this one was still active, looked nice and was published ...... one more to the sink!
BG7 is good in that it aligns known proteins to the genome, so you are more likely to get the correct start codon. Prokka 1.x predicts genes ab initio and sometimes gets it wrong. Prokka 2.x will likely incorporate both ideas.
I tried to install but did not find any info about it nor on how to use the tutorial data. Is BG7 one of these orphan projects that dies after publication or is it functional? Any help welcome!