bg7 / BG7

bacterial genome annotation system
bg7.ohnosequences.com
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Is this code still supported? #48

Closed splaisan closed 5 years ago

splaisan commented 5 years ago

I tried to install but did not find any info about it nor on how to use the tutorial data. Is BG7 one of these orphan projects that dies after publication or is it functional? Any help welcome!

splaisan commented 5 years ago

I tried the following:

bin/bg7 -n bg7_example \ -g bg7_example_input_files/escherichia_coli_o104_h4_str._ty-2482_1_supercontigs.fasta \ -p bg7_example_input_files/EHEC_ReferenceProteins_17_08_2012.fasta \ -r bg7_example_input_files/EHEC_Reference_RNAs.frn \ -c bg7_example_input_files/genetic_code.txt \ -o bg7_example \ -G bg7_example_input_files/GenBankExternalData.xml

logging your params to /opt/biotools/BG7/bg7_example/params.log
it looks like this machine has 88 cores, will use that for blast settings
creating RNAs blast db
RNA blast db created: /opt/biotools/BG7/bg7_example/bg7_example.rnas.db
if you want, you can check makeblastdb log: /opt/biotools/BG7/bg7_example/bg7_example.rnas.db.log
running blastn: RNAs vs genome sequence
done! will do a basic results check now
checking if blast xml output file looks ok...
$ ll bg7_example/
total 128K
drwxr-xr-x 2 u0002316 domain users 4.0K Aug 14 11:30 .
drwxr-xr-x 9 u0002316 domain users 4.0K Aug 14 11:29 ..
-rw-r--r-- 1 u0002316 domain users  69K Aug 14 11:30 bg7_example_RNA_blastn.xml
-rw-r--r-- 1 u0002316 domain users  361 Aug 14 11:30 bg7_example.rnas.db.log
-rw-r--r-- 1 u0002316 domain users  22K Aug 14 11:30 bg7_example.rnas.db.nhr
-rw-r--r-- 1 u0002316 domain users 2.0K Aug 14 11:30 bg7_example.rnas.db.nin
-rw-r--r-- 1 u0002316 domain users  12K Aug 14 11:30 bg7_example.rnas.db.nsq
-rw-r--r-- 1 u0002316 domain users  529 Aug 14 11:30 params.log

this does not look like completed to me ...

tseemann commented 5 years ago

You can always try Prokka https://github.com/tseemann/prokka :-)

splaisan commented 5 years ago

Thanks @tseemann; I was hoping this one was still active, looked nice and was published ...... one more to the sink!

tseemann commented 5 years ago

BG7 is good in that it aligns known proteins to the genome, so you are more likely to get the correct start codon. Prokka 1.x predicts genes ab initio and sometimes gets it wrong. Prokka 2.x will likely incorporate both ideas.