I didn't realise from the help that this tool is designed for and defaulting to animal data. When I tried with fungal data then all of the blast matches were filtered out, looking at the mitohifi source then this seems to be due to the animal NUMT criteria.
tools/mitohifi/mitohifi.xml
I didn't realise from the help that this tool is designed for and defaulting to animal data. When I tried with fungal data then all of the blast matches were filtered out, looking at the mitohifi source then this seems to be due to the animal NUMT criteria.
It might be a good idea to highlight this information and maybe expose the -a parameter to choose animal, plant, or fungi (see https://github.com/marcelauliano/MitoHiFi/blob/1683dbc6c0d0a0d4ea7b0aa5aa1e1701b63cb6aa/src/mitohifi.py#L57).