search

bihealth / digestiflow-demux

:spaghetti: Digestiflow Demultiplexing Tool
MIT License
2 stars 4 forks source link

Handling of runs with UMIs #2

Closed messersc closed 5 years ago

messersc commented 5 years ago

To me, it looks like there is no good and robust way to decide if a run is dual barcode or barcode + UMI from the flowcell info.

Current test case is https://flowcells.bihealth.org/flowcells/22875de2-0f5f-42f6-84ec-5eb085f6c02f/flowcell/a988e5e7-48db-4902-ad82-5d3e13f642ec/

I changed the current_reads from 126T8B10B126T to 126T8B10M126T - 10B to 10M. Demultiplexing with picard yields R1, R2, barcode_1 and index_1 files.

How should we handle this in general? Do you think this one manual intervention is necessary?

holtgrewe commented 5 years ago

We will introduce an additional field in digestiflow-web for overriding this.

holtgrewe commented 5 years ago

Added demux_reads to override planned_reads