bihealth / seasnap-pipeline

SeA-SnaP: (Se)q (A)nalysis (Sna)kemake (P)ipeline
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RNA-SeQC strandness issue #25

Closed AndranikIvanov closed 1 year ago

AndranikIvanov commented 1 year ago

rnaseqc and rseqc have opposing results when running on stranded data. The issue seem to be coming from rna-seqc: we need to understand what 'fr' and 'rf' specify exactly. as of now the pipeline runs rf for the reverse and fr for the forward stranded protocols