Closed billzt closed 2 years ago
Now it simply replaces degenerate bases with a random normal base. However it is not suitable for primers with multiple degenerate bases since it would result in too many mismatches and lose the true amplicon
Now it simply replaces degenerate bases with a random normal base. However it is not suitable for primers with multiple degenerate bases since it would result in too many mismatches and lose the true amplicon