update sequencers i5 reversecomp details in samplesheet generation info in notebooks.

biocore / metagenomics_pooling_notebook

Jupyter notebooks to assist with sample processing

MIT License

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update sequencers i5 reversecomp details in samplesheet generation info in notebooks. #148

Closed RodolfoSalido closed 9 months ago

RodolfoSalido commented 12 months ago

Looks like the information in the notebooks is outdated and could generate confusions regarding i5 reverse compliment requirements. Underlying code in metapool is accurate, so just need to correct jupyter notebook markdown.

Might be pertinent to add NovaSeq X as another sequencing platform, but only if there is a distinction between NovaSeq 6000 and NovaSeq X in regards to i5 orientation, which I'm still investigating.

RodolfoSalido commented 12 months ago

NovaSeq 6000 and NovaSeq X should have same i5 index orientation, so no need to further disambiguate between both when generating sample sheets (yet)