biocore / mg-scripts

Knight Lab internal Metagenomic processing scripts for demultiplexing, QC and host removal
BSD 3-Clause "New" or "Revised" License
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add KATHAROseq samples to SIFs. #128

Open charles-cowart opened 8 months ago

charles-cowart commented 8 months ago

Samples in pre-prep files beginning w/KATHARO will be added to SIFs just like BLANKs are. Based on a comparison between the columns in the pre-prep files and the SIFs, I don't see any overlap where additional column values should be populated using the pre-prep file. Hence, the KATHARO rows will be populated w/the same defaults that BLANKs are.

No tests were affected, and no tests were broken due to this change. That being said, it's relatively straightforward and trivial and I think we can dispense with an additional test for now.

wasade commented 8 months ago

I don't think the defaults are the same as these are positive not negative controls. And, it's necessary that the values like cell count are part of metadata

charles-cowart commented 8 months ago

I don't think the defaults are the same as these are positive not negative controls. And, it's necessary that the values like cell count are part of metadata

@wasade ty! I'll send Gail an email in the morning and show her what we're populating the blanks with, the columns in the SIFs, and she can let me know what to fill in.

coveralls commented 8 months ago

Pull Request Test Coverage Report for Build 8024854693

Details


Totals Coverage Status
Change from base Build 8024026518: 0.008%
Covered Lines: 2367
Relevant Lines: 2678

💛 - Coveralls
wasade commented 8 months ago

Thanks! And please remember that the individual KatharoSeq controls samples will differ from each other, and that those differences are necessary to interpret the data.

charles-cowart commented 8 months ago

Just to update this conversation, I emailed Gail and MacKenzie regarding this issue and she's going to provide information today and Monday to address it.

wasade commented 8 months ago

Great, thanks!

On Feb 23, 2024, at 12:44 PM, Charles Cowart @.***> wrote:

Just to update this conversation, I emailed Gail and MacKenzie regarding this issue and she's going to provide information today and Monday to address this issue.

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