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biocore / qiime

Official QIIME 1 software repository. QIIME 2 (https://qiime2.org) has succeeded QIIME 1 as of January 2018.
GNU General Public License v2.0
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Help with ClustalW.. #2227

Open AlliKoehle opened 4 years ago

AlliKoehle commented 4 years ago

I am trying to run an alignment with ClustalW, but I keep receiving this error...

Traceback (most recent call last): File "/macqiime/anaconda/bin/align_seqs.py", line 211, in main() File "/macqiime/anaconda/bin/align_seqs.py", line 208, in main log_path=log_path) File "/macqiime/anaconda/lib/python2.7/site-packages/qiime/align_seqs.py", line 123, in call log_path=log_path, *args, *kwargs) File "/macqiime/anaconda/lib/python2.7/site-packages/qiime/util.py", line 259, in call result = self.getResult(args, **kwargs) File "/macqiime/anaconda/lib/python2.7/site-packages/qiime/align_seqs.py", line 117, in getResult result = module.align_unaligned_seqs(seqs, params=params) File "/macqiime/anaconda/lib/python2.7/site-packages/bfillings/clustalw.py", line 584, in align_unaligned_seqs app = Clustalw(InputHandler='_input_as_multiline_string',params=params) File "/macqiime/anaconda/lib/python2.7/site-packages/burrito/util.py", line 201, in init self._error_on_missing_application(params) File "/macqiime/anaconda/lib/python2.7/site-packages/burrito/util.py", line 468, in _error_on_missing_application "Is it in your path?" % command) burrito.util.ApplicationNotFoundError: Cannot find clustalw. Is it installed? Is it in your path?

This is my first time using Qiime, so I unsure of what I should to doing to resolve this error. Can anyone help with this?