biocore / qiime

Official QIIME 1 software repository. QIIME 2 (https://qiime2.org) has succeeded QIIME 1 as of January 2018.
GNU General Public License v2.0
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Problems using count_seqs.py with joined reads #2231

Open andso17 opened 2 years ago

andso17 commented 2 years ago

I am pretty new to using these tools, so I hope I am not an inconvenience.

I am having problems with processing of an outputfile of joined reads, processed with the fastq-join from ea-utils. When running the following command I get an error, that seems to originate from failed import of files:

count_seqs.py -i join

(here 'join' is the name of the file of the joined, paired reads generated via ea-utils)

The error message looks like this:

Traceback (most recent call last): File "/data/miniconda3/envs/qiime/bin/count_seqs.py", line 13, in from qiime.util import (parse_command_line_parameters, File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/qiime/util.py", line 49, in from skbio.util import remove_files, create_dir File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/init.py", line 15, in import skbio.io File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/io/init.py", line 309, in import_module('skbio.io.clustal') File "/data/miniconda3/envs/qiime/lib/python2.7/importlib/init.py", line 37, in import_module import(name) File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/io/clustal.py", line 123, in from skbio.alignment import Alignment File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/alignment/init.py", line 230, in from ._alignment import Alignment, SequenceCollection, StockholmAlignment File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/alignment/_alignment.py", line 21, in from skbio.stats.distance import DistanceMatrix File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/stats/distance/init.py", line 193, in from ._base import (DissimilarityMatrixError, DistanceMatrixError, File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/skbio/stats/distance/_base.py", line 17, in from IPython.core.pylabtools import print_figure File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/IPython/init.py", line 48, in from .core.application import Application File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/IPython/core/application.py", line 23, in from traitlets.config.application import Application, catch_config_error File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/traitlets/config/init.py", line 6, in from .application import * File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/traitlets/config/application.py", line 17, in from decorator import decorator File "/data/miniconda3/envs/qiime/lib/python2.7/site-packages/decorator.py", line 162 print('Error in generated code:', file=sys.stderr) ^

I would be very grateful for any help on the matter

andso17 commented 2 years ago

Sorry, the arrow (last line) is supposed to point at '='. Below the arrow, this error-message is printed: "SyntaxError: invalid syntax"