I am using sambamba depth region to look ar read coverage at 10 bp intervals. I get an error message saying " sambamba-depth: couldn't open file or find reference" about my .bed file. It has three columns, one for chromosome, one for start of interval and one for stop of interval.
I don't understand what's wrong here.
I had a similar issue, if your bed file has a header line, i.e. "#chrom start stop", try removing that. That seemed to be what was causing the error for me.
Hi
I am using sambamba depth region to look ar read coverage at 10 bp intervals. I get an error message saying " sambamba-depth: couldn't open file or find reference" about my .bed file. It has three columns, one for chromosome, one for start of interval and one for stop of interval. I don't understand what's wrong here.
I have checked that all the pathways are correct.
Thank you