10.1534/genetics.120.303085

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085 @kyook New publication uploaded.

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085 @kyook started curating

[kyook]

WBPaper00059266

[kyook]

new alleles and transgenes, suppressor alleles are written as compound alleles, will give each suppressor its own variation

[kyook]

typo - sup-1 should be sups-1 and linked "WGS of sup-1(xn7) and sups-1(xn20) are deposited at NCBI as Sequence Read Archive data (xn7: SRR9969397; xn20: SRR9988268). Supplemental material available at figshare: https://doi.org/10.25386/genetics.9637079."

[kyook]

xn7 after forward slash not linked: genotype: xn7/xn7 (positive control):

[kyook]

can't link xn8 in this string: pkc-3(ne4250xn8)

[kyook]

Italicized and saved format change for 'xnIs131', but doesn't stay italicized in texture

[kyook]

typo? xn87? pkc-3(ne4250) worms or N2 worms were injected using a crRNA with target homology sequence 5′-AGCTTTCGAAGCATTAGTCT-3′ and ssDNA oligo 5′-GTAATCAAGCATACCTCGCGGAGCTTTCGAAGCATTAGTCTGAACTGAATGCGTACAGCGCTAAACAGAT-3′. To create sups-1(xn87[sups-1::yfp]), a plasmid derived from pDD122 (​​​​​​Dickinson et al. 2013) expressing Cas9 and a sgRNA with target homology sequence 5′-GATACGGATGACTTTAATAGG-3′, and repair template derived from pSA122 (​​​​​​Armenti et al. 2014b) containing yfp and 1.5 kb homology arms, were injected into unc-119(ed3) worms as described (​​​​​​Dickinson et al. 2013). A strain carrying the insertion was genotyped by sequencing PCR product amplified with primers 5′-CTTTAATAGGCGACACTTTGATGATGTCTC-3′ and 5′-ATGTCAGAAGTGATTTGGTATGCCGTACTC-3′.

[kyook]

trying to get the WBVar ID for ne4250, name server not working, connection iffy. Had retrieved one earlier but now do not see it. Had linked this entity with different WBVarID's earlier - need to remove it from the lexicon. "WBPaper00059266 To identify intragenic suppressor mutations, the complete pkc-3 genomic locus was amplified using primers 5′-CAGTTTCGCAGTTTTCGTTCAG-3′ and 5′-CCACGAGTTAGTGTGTCATAG-3′ (3803 bp) and sequenced. In addition to the H390Q mutation in the kinase domain, pkc-3(ne4250) contains an E9K missense mutation near the N-terminus outside of any known functional domains."

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085

Link validation report

Validated Links

Invalidated Links

• xn37
• xn20
• xn7
• sups-1
• it309
• xn8
• xnIs131
• sym-1
• PKC-3
• pkc-3
• dd21
• lgl-1
• sIs10089
• cn64
• TMUE_0000000498
• par-6
• mn601
• ne4246
• dpy-10
• mec-8
• naIs8
• ed3
• let-7
• PPA10264
• ok544
• unc-119
• lin-28
• e398
• cdc-42
• lin-4
• ne4250
• xn87
• xn84

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085 @kyook marked publication ready for publisher review. @Genetics-Database The article is now available on your server. @kyook

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085 @kyook started curating

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085

Link validation report

Validated Links

Invalidated Links

• pkc-3
• PKC-3
• xn8
• e398
• ne4250
• sups-1
• xn20
• lgl-1
• unc-119
• mn601
• xnIs131
• par-6
• dd21
• xn7
• it309
• lin-4
• sym-1
• let-7
• xn37
• lin-28
• naIs8
• cdc-42
• sIs10089
• ed3
• PPA10264
• dpy-10
• mec-8
• sup-1
• cn64
• ne4246
• TMUE_0000000498
• xn87
• ok544
• xn84

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085 @kyook marked publication ready for publisher review. @Genetics-Database The article is now available on your server. @kyook

[kyook]

need to remove links in ::pkc-3(xnIs131)

[bioentity-bot]

https://bioentity.link//#/publication/10.1534/genetics.120.303085 @kyook started curating