Open Alipe2021 opened 2 years ago
Hi guys,
When I ran the command:
CIRIquant --config circ_quant_cfg.yaml -1 sampleA.R1.fq.gz -2 sampleA.R2.fq.gz --bam sampleA_sorted.bam -o ./sampleA -p sampleA -t 8 --bed CircRNAs.bed -e CIRIquant.log
It got an error as follows:
[Wed 2022-03-02 20:34:12] [INFO ] Detecting FSJ reads from genome alignment file Traceback (most recent call last): File "/opt/miniconda3/envs/CIRIquant_env/bin/CIRIquant", line 11, in <module> load_entry_point('CIRIquant==1.1.2', 'console_scripts', 'CIRIquant')() File "/opt/miniconda3/envs/CIRIquant_env/lib/python2.7/site-packages/CIRIquant-1.1.2-py2.7.egg/CIRIquant/main.py", line 184, in main out_file = circ.proc(log_file, thread, bed_file, hisat_bam, rnaser_file, reads, outdir, prefix, anchor, lib_type) File "/opt/miniconda3/envs/CIRIquant_env/lib/python2.7/site-packages/CIRIquant-1.1.2-py2.7.egg/CIRIquant/circ.py", line 656, in proc bsj_reads, fsj_reads = proc_genome_bam(hisat_bam, thread, circ_info, cand_bsj, anchor, circ_dir) File "/opt/miniconda3/envs/CIRIquant_env/lib/python2.7/site-packages/CIRIquant-1.1.2-py2.7.egg/CIRIquant/circ.py", line 434, in proc_genome_bam tmp = job.get() File "/opt/miniconda3/envs/CIRIquant_env/lib/python2.7/multiprocessing/pool.py", line 572, in get raise self._value ValueError: start out of range (-1)
Can any one help me to solve this problem. Thanks.
Hi @Alipe2021, could you try running CIRIquant without the --bam parameter? It seems that the sampleA_sorted.bam is not generated correctly.
--bam
Hi guys,
When I ran the command:
It got an error as follows:
Can any one help me to solve this problem. Thanks.