Closed mmaitenat closed 2 years ago
Hi @mmaitenat, the '--canonical' option is not effective. Considering the high-error rate of nanopore reads, it's more appropriate to use defined splicing signals rather than perform de novo alignment. Here's the instructions to modify splice signal sequences in CIRI-long: https://ciri-cookbook.readthedocs.io/en/latest/CIRI-long_2_usage.html#using-non-canonical-splice-signals
Hi again,
I would like CIRI-long not to rely on the canonical GT/AG splice signal only. However, when I run the tool both with and without the
--canonical
argument, the results are the same. If I understood correctly, the--canonical
option is always set to TRUE. Is it possible to disable it so that non-canonical ones are also considered?Thanks,
Maitena.