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bioinfo-biols / CIRI-long

Circular RNA Identification for Nanopore Sequencing
https://ciri-cookbook.readthedocs.io
MIT License
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Confusing results that circ_len < 0 #8

Closed gnilihzeux closed 2 years ago

gnilihzeux commented 2 years ago

Dear author,

Threre are some confusing results as the pic: 企业微信截图_16372074866060

BTW, there are some other items which consused me

  1. The * after splice_site, what's that
  2. what is equivalent_seq (although your manual presents a little message)

Thanks.

Kevinzjy commented 2 years ago

Hi, @gnilihzeux these should be false-positive results from erroneous reads. You could filter this out from the final result.

Besides, "*" after splice site refers to novel splice sites that are not annotated in the reference GTF, and equivalent_seq are the repetitive sequences in the back-splice junction region for debugging purposes.

gnilihzeux commented 2 years ago

That's very helpful. Thank you very much.