How to detect Alternative Splicing events ?

[gnilihzeux]

Dear author,

Could you please share your strategy that how to detect alternative splicing events ? There seems to be no clue in the paper.

What I can think of is comparing circRNA exons model with known gene model. There is a schematic diagram of Exon Skipping below

Gene       -----------IIIIIIIIIIIIIIIIIIIII----------IIIIIIIIIIIIIIIIIIIII----------IIIIIIIIIIIIIIIIIIIIIIIII-------------
circRNA               IIIIIIIIIIIIIIIIIIIII                                         IIIIIIIIIIIIIIIIIIIIIIIII

But it's hard for me to achieve this especially for reads assignment to each exon.

So, is there any tool to implement this work, just like rMATS using in next-generation RNA-seq ?

Thanks a lot.

[Kevinzjy]

The alternative splicing events are annotated by customized scripts. We could release a future version to output the ratio of different isoforms in the same BSJ event.

[gnilihzeux]

Thanks. That would be great.