bioinfoUGR / sRNAtoolbox

small RNA analysis programs: standalone jar files (sRNAbench, sRNAde, etc), manuals and Docker image
MIT License
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Format for spike-in sequences #28

Open kartikshankarcu opened 10 months ago

kartikshankarcu commented 10 months ago

Hi I am trying to include spike-in sequences for small RNA analysis. The libraries were made using the Qiaseq UMI method. I included the spike-in sequences as a fasta file or as a csv but both ended with errors. The same fastq files run fine with out the spike-in option enables. What is the format in which the spike-in sequences should be specified?

thanks

bioinfoUGR commented 10 months ago

please provide a jobID of your failed attempt, example input files and example spike-in files.