Hi
I am trying to include spike-in sequences for small RNA analysis. The libraries were made using the Qiaseq UMI method. I included the spike-in sequences as a fasta file or as a csv but both ended with errors. The same fastq files run fine with out the spike-in option enables. What is the format in which the spike-in sequences should be specified?
Hi I am trying to include spike-in sequences for small RNA analysis. The libraries were made using the Qiaseq UMI method. I included the spike-in sequences as a fasta file or as a csv but both ended with errors. The same fastq files run fine with out the spike-in option enables. What is the format in which the spike-in sequences should be specified?
thanks