Arkadiy-Garber
commented
1 year ago For some context, this was the preceding guppy run, which finished without issue:
guppy_basecaller -i barcode12/ -r -s barcode12/ --flowcell FLO-MIN114 --kit SQK-NBD114-24 --cpu_threads_per_caller 16 --resume --as_cpu_threads_per_scaler 16 --num_barcoding_threads 16 --bam_out
Getting the following output. Some help would be appreciated.
(deepsignalenv) MAB@Axceleron-WKS:~/2748_NP_methylation/fast5_pass$ tombo preprocess annotate_raw_with_fastqs --fast5-basedir single_barcode12/ --fastq-filenames barcode12/barcode12.guppy.fastq[16:00:39] Preparing reads and extracting read identifiers. 100%|██████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████████| 110340/110340 [03:07<00:00, 587.69it/s] [16:03:48] Annotating FAST5s with sequence from FASTQs. ** WARNING ** Some FASTQ records contain read identifiers not found in any FAST5 files or sequencing summary files. 0it [00:01, ?it/s] [16:03:50] Added sequences to a total of 0 reads.
(deepsignalenv) MAB@Axceleron-WKS:~/2748_NP_methylation/fast5_pass$Thanks, Arkadiy