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biosails / pheniqs

Fast and accurate sequence demultiplexing
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IO error #36

Closed austenapigo closed 2 years ago

austenapigo commented 2 years ago

Hi, I am new to pheniqs and receiving the following error when trying to demultiplex Illumina reads with i5-i7 pairings on my campus computer cluster. I am getting all the .fastq.gz files I would like as output, but am unable to unzip them. I am not sure if that is due to the error. Thank you for any help you can provide.

IO error : failed to open ./ST2_8_07_H.R1.fastq.gz for writing with error code 24 Full Command: pheniqs mux --config pheniqs_ex.json Memory (kb): 769164

SWAP (freq): 0

    # Waits (freq):                    4063
    CPU (percent):                     36%
    Time (seconds):                    59.77
    Time (hh:mm:ss.ms):                0:59.77
    System CPU Time (seconds):         5.47
    User   CPU Time (seconds):         16.24

[Linux@vaughan sge.pheniqs.ex]$ pheniqs --version pheniqs version git-HEAD zlib 1.2.11 bzlib 1.0.8 xzlib 5.2.5 libdeflate 1.6 rapidjson 1.1.0 htslib 1.11

json file attached pheniqs_ex.json.txt

austenapigo commented 2 years ago

Hi again, I think this was an issue with my campus cluster and the memory setting. I ran the same .json with a smaller barcode set and it worked perfectly.