biostars / biostar-handbook

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Issue with genes.gff file in section 104.6 "What else can I do to refine the peaks?" #143

Open ChristianKKelley opened 3 years ago

ChristianKKelley commented 3 years ago

Hello,

In this part, there is a command

bedtools flank -g refs/sc.fa.fai -l 1000 -r 0 -s -i refs/genes.gff > flanked.gff

A small issue is that the file that I think it first references is saccer3.fa.fai, not sc.fa.fai (as that doesn't exist so far, given instructions). Second, there aren't instructions to obtain genes.gff (at least as far as I'm aware, I haven't found any in the ChIP-Seq section); I went here, via the link from the handbook and clicking on the "Genome" link under "Related Information", and obtained the gff file there. However, running the above command gives this error:

ERROR: chrom "NC_001133.9" not found in genome file. Exiting.

Wondering if you would be able to include where the gff file comes from