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bjartelund / Thermoslope

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Consider single experiment per temperature approach #5

Open bjartelund opened 4 years ago

bjartelund commented 4 years ago

On the estimation errors of KM and V from time-course experiments using the Michaelis–Menten equation Wylie Stroberg, Santiago Schnell bioRxiv 068015; doi: https://doi.org/10.1101/068015 Now published in Biophysical Chemistry doi: 10.1016/j.bpc.2016.09.004

bjartelund commented 4 years ago

Some clear drawbacks with the approach: You would have to run the individual experiments much longer In my case, you reach a saturation-point where the detector is no longer capable of measuring the signal.

bjartelund commented 4 years ago

thermofit-gradient does use the absorbance to calculate concentration, could be extended to the other script as well