I would like to use ROVER for amplicon deep sequencing to increase sensitivity. I have a couple of questions you might be able to answer. Those questions do not address software issues but are referring to methods. However, I think they might be relevant for other users of ROVER.
· How did you prep the library?
· Which amlicon size did you choose?
· Which Alligner Pope did you use?
· Did you use PICARD to filter PCR duplicates before ROVER analysis?
It would be really great if you could help me. Thanks,
Hi everybody,
I would like to use ROVER for amplicon deep sequencing to increase sensitivity. I have a couple of questions you might be able to answer. Those questions do not address software issues but are referring to methods. However, I think they might be relevant for other users of ROVER.
· How did you prep the library?
· Which amlicon size did you choose?
· Which Alligner Pope did you use?
· Did you use PICARD to filter PCR duplicates before ROVER analysis?
It would be really great if you could help me. Thanks,
Stefan