blaxterlab / blobology

Tools for making blobplots or Taxon-Annotated-GC-Coverage plots (TAGC plots) to visualise the contents of genome assembly data sets as a QC step (NO LONGER MANTAINED)
MIT License
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Separating forward and reverse reads after "separate_reads.bash" #7

Open mofraz opened 8 years ago

mofraz commented 8 years ago

I was wondering if there is a way to retain the stranded information for reads after deciding on which contigs to keep, and extracting the reads that align to them. The result of this process is a single file for each paired end library, but I am wondering how this will affect downstream differential gene expression analyses, if at all.