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borenstein-lab / MUSiCC

MUSiCC: A marker genes based framework for metagenomic normalization and accurate profiling of gene abundances in the microbiome
BSD 3-Clause "New" or "Revised" License
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Running MUSiCC With Metagenomic Data #4

Open dcdanko opened 6 years ago

dcdanko commented 6 years ago

I was wondering if you had any guidance for running MUSiCC with taxonomic profiles from metagenomic data rather than OTUs?

If this is not possible do you recommend running PiCRUST on samples before feeding them into MUSiCC? Other tools to obtain OTUs/KOs from metagenomic data?

Edit: reading through the forum it seems like you recommend using Humann2 to estimate KO abundances for metagenomes?