Burrito Unable to use a taxonomy abundance table

[diegoibt]

Hi!

Im trying to use burrito to analyze a dataset obtained with picrust2; when I try the most simple way to use Burrito with Taxonomic abundances: (OTU abund table), Custom taxon IDs with no hierachy, and Taxonomy-function linking method: Function attribution table (from picrust2), run smoothly, but I need to analyze my data with a hierarchical taxonomy (Custom Taxonomy file), Burrito says that I have OTUs in the contribution table, but are not present in the OTU table, with the same data, and that's not accurate, since I have the same data. I already check all headers, names, ids etc of my tables and are accurate. Can you help me with this issue?

I attached my files and error messages

https://drive.google.com/drive/folders/1Ie_Q6nB-RBgEBpSsFn83naBvnh0646-T?usp=sharing

[engal]

Hi,

Sorry to hear you're running into an issue. Unfortunately, I tried out the attached files and could not reproduce the error you posted. For reference, here's the output I got when I used your custom taxonomy file:

This was done with all default options on the upload the page. The only things I did were:

1. Upload the taxonomic abundance table
2. Upload the custom taxonomic hierarchy
3. Upload the attribution table.

When you tried, did you make any other changes on the upload page? If so, that information might help me figure out what is causing your issue.

[diegoibt]

Thank you, Engal

With this dataset works smoothly, but when I try with a bigger dataset, shows and "Disconnected from the server", "reload"

message and reload... and thats all, Burrito not continues with my analysis.

Can you help me with this issue?

I attached my files and error messages

https://drive.google.com/drive/folders/1Ie_Q6nB-RBgEBpSsFn83naBvnh0646-T?usp=sharing

[engal]

Hi,

I think I found the issue. Could you add a non-empty column header for the sample ID column in your metadata file (e.g. "sample") and try again?