The following OTU_IDs are in the OTU table but are not present in the PICRUSt 16S normalization table

[FelixGiraud]

Hello, I'm really enjoying Burrito and have managed to run it with one dataset. However, i've been trying to upload a second dataset many times already without success.

Here is my OTU table: otu_well_formated.txt My metadata table: metadata_well_formated.txt And my metagenome predictions: pred_burrito_ok.txt

I'm really confused since I tried to switch the GG ID's of this dataset with the IDs of my first dataset and Burrito worked just fine. If you have any idea I would really appreciate. Thank you.

[engal]

Hi,

How did you pick these OTUs? Looking at the gg_13_5_otus reference set (see here), the OTUs in your table do not appear to have associated representative sequences.

[FelixGiraud]

I used this command in order to generate my table:

qiime2 vsearch cluster-features-closed-reference \ --i-table table.qza \ --> table genrated with Dada2 --i-sequences rep-seqs.qza \ --> input sequences --i-reference-sequences 99_otus.qza \ --> GG_13_5 sequences. --p-perc-identity 0.99 \ --o-clustered-table table-cr-99.qza \ --o-clustered-sequences rep-seqs-cr-99.qza \ --o-unmatched-sequences unmatched-cr-99.qza

Then I converted the file to a tab delimited format. I can definitly find matches between the gg_13_5_taxonomy and the gg_13_5 fasta using the link you provided.

I'm really confused, I'm probably missing something but I can't figure out what it is...

[engal]

Where did you get your 99_otus.qza file from? The IDs may exist in the taxonomy file, but there is no associated representative sequence the Greengenes 99_otus.fasta file that contains the representative sequences for 99% identity clustering (which is found in the gg_13_5_otus archive on the page I linked).

[FelixGiraud]

Looks like this is it. I used the gg_13_5.fasta.gz instead of the gg_13_5_otus.gz file. I'll give it a try and close the subject if the problem is solved. Thank you very much for your help.

[FelixGiraud]

Works perfectly. Thank you 👍

[stepnap]

Hii, this is my first time using BURRITO. But when I try to use the tools I have this error: The following OTU_IDs are in the OTU table but are not present in the PICRUSt 16S normalization table: .... Here my OTU table, taxonomy and metadata. taxonomy2.txt OTU.txt metadata.txt If you have any idea I would really appreciate. Thank you :).

[stepnap]

Is possible that burrito work well only with OTU and not with AVS ?