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borenstein-lab / burrito

A visualization tool for exploratory data analysis of metagenomic data
https://elbo-spice.gs.washington.edu/shiny/burrito/
GNU General Public License v3.0
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Requeried files - picrust2 #28

Open debC23 opened 2 years ago

debC23 commented 2 years ago

Hi Users! I´m a Burrito´s begginer and I am confussed about the infiles I have to upload. I have data from 16S sequences. They were analized by qiime2 (ASVs and Silva database). Then, I used picrust2. In this case, I selected KO-metagenome option. I have a list of files that I tried to run with Burrito, but I can not do it. The error message says Disconnected server. Reload, or something that. Internet connection works well. I read the instructions and I used :

Taxonomic abundances: table biom (from Qiime2)

Taxonomy-function linking method: Function attribution table: pred_metagenome_contrib.legacy

I attach these files. I hope you can guide me

Thanks in advance,

pred_metagenome_contrib.legacy.tsv.gz

table-biom.zip

Deb!

engal commented 2 years ago

Hi Deb,

Since you're using ASVs rather than Greengenes OTUs, Burrito's default taxonomy data is not compatible with your data. Instead, you'll also need to provide a custom taxonomy file (see example on the upload page for formatting help).

Hope that helps!