jwinter6
commented
6 years ago HI Katharina,
right now this is not possible, at least two samples for either the untreated or treated population is required. Since the analysis of data without any biological replicates at all is not recommended, I only implemented a workaround to allow a single replicate for one of the groups. Unfortunately, this cannot be changed since some degrees of freedom are required.
What can be done (and is often done):
- a single replicate for day 0 (often the library sequencing)
- two replicates for day X
But apart from that I suggest to just be patient until the replicates are there :)
cheers Jan
Hi,
I would really like to use your tool to analyze my first round of samples. I do not have their biological replicates yet and when I tried running the analysis, it failed when trying to run edgeR. Is there a way of selecting only the algorithms that do not require replicates?
Best, Katharina