boutroslab / CRISPRAnalyzeR

CRISPRAnalyzeR: interactive analysis, annotation and documentation of pooled CRISPR screens
GNU General Public License v2.0
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CRISPRAnalyzeR crashed for the sample data #37

Closed xuguorong2016 closed 6 years ago

xuguorong2016 commented 6 years ago

Hi Jan,

I installed CRISPRAnalyzeR with docker on my local mac laptop without problem. But I got the crash issue when I tried to load the TRAIL_Resistance sample data.

screen shot 2018-01-19 at 11 25 26 pm

Then I access the demo website and got the same issue. http://crispr-analyzer.dkfz.de/

From the log files, I did not find any problem. Do you have any ideas? logs.zip

Your response would be really appreciated!

Guorong

jwinter6 commented 6 years ago

Hi Guorong, thanks for your help :) If the app greys out it lost the connection to your browser. This can also be an issue with the app, that is true. I just tried the sample data using the online demonstration and it worked for me.

I selected EXAMPLE Trail resistance in the library selection and uploaded the following files: image

can you please give it another try?

I also checked your logs and there might be a small issue when the shiny session is initiated, I will have a look into it.

Best Jan

xuguorong2016 commented 6 years ago

Hi Jan,

Thanks a lot! I just selected the pre-made screen library EXAMPLE Trail resistance and it worked well. But I downloaded the fasta file from the website to load back, it greyed out. I think it is possible the fasta file issue. I have attached the fasta file and please try it.

Thanks, Guorong pilotscreen_TRAIL.fasta.zip

xuguorong2016 commented 6 years ago

Hi Jan,

I think I figured out what's going on. When I loaded the downloaded fasta file into CRISPRAnalyzeR I did not check the type of screen library. So the CRISPRAnalyzeR got the crash issue. After I selected the custom option and then uploaded the fasta file, it works fine.

Thanks, Guorong

jwinter6 commented 6 years ago

Glad it works now :)

best, Jan