All count of sgRNAs are 0 while more than 90% reads mapped to library

boutroslab / CRISPRAnalyzeR

CRISPRAnalyzeR: interactive analysis, annotation and documentation of pooled CRISPR screens

GNU General Public License v2.0

80 stars 31 forks source link

All count of sgRNAs are 0 while more than 90% reads mapped to library #57

Open frankzelph opened 5 years ago

frankzelph commented 5 years ago

I used a custom library. After uploading the library file and fastq.gz data files, "Upload and Check Files" can proceed successfully. But when I click " Review your data", it shows good mapping ratios of all samples and good fastq quality. Could you help me with this issue? Thanks and sorry for taking your time.

mirabai-cuenca commented 5 years ago

It happens exactly the same to me with a custom library (Yusa library from Addgene), did you manage to solve the issue?