Closed cindy12-gao closed 3 years ago
HI sorry about this. Have you recently updated cellfinder? If so, can you also update cellfinder-core
(pip install cellfinder-core -U
)?
If this doesn't work, could you try installing cellfinder into a new conda environment?
Hi! after updated the cellfinder-core, it can recognized the command. but there is an error, it can`t produce the ch1 file. and the error log like this.
发件人:Adam Tyson @.> 发送时间:2021年4月28日(星期三) 17:30 收件人:brainglobe/cellfinder @.> 抄 送:高辛未 @.>; Author @.> 主 题:Re: [brainglobe/cellfinder] [BUG]cellfinder training --cellfinder==0.4.12 (#174)
HI sorry about this. Have you recently updated cellfinder? If so, can you also update cellfinder-core (pip install cellfinder-core -U)? If this doesn't work, could you try installing cellfinder into a new conda environment? — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub, or unsubscribe.
(cellfinder) C:\Users\CIBR-ImFacility-WS2>cellfinder_train -y D:\AM_tiff\output\points\training.yml -o D:\AM_tiff\output\points --epochs 100
2021-04-28 17:46:23 PM - INFO - MainProcess fancylog.py:267 - Starting logging
2021-04-28 17:46:23 PM - INFO - MainProcess fancylog.py:269 - Multiprocessing-logging module found. Logging from all processes
2021-04-28 17:46:24 PM - DEBUG - MainProcess tpu_cluster_resolver.py:36 - Falling back to TensorFlow client; we recommended you install the Cloud TPU client directly with pip install cloud-tpu-client.
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - (private) matplotlib data path: c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\site-packages\matplotlib\mpl-data
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - matplotlib data path: c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\site-packages\matplotlib\mpl-data
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - CONFIGDIR=C:\Users\CIBR-ImFacility-WS2.matplotlib
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1480 - matplotlib version 3.3.3
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1481 - interactive is False
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1482 - platform is win32
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1483 - loaded modules: ['sys', 'builtins', '_frozen_importlib', '_imp', '_thread', '_warnings', '_weakref', 'zipimport', '_frozen_importlib_external', '_io', 'marshal', 'nt', 'winreg', 'encodings', 'codecs', '_codecs', 'encodings.aliases', 'encodings.utf_8', '_signal', 'encodings.latin_1', 'io', 'abc', '_abc', 'site', 'os', 'stat', '_stat', '_collections_abc', 'ntpath', 'genericpath', 'os.path', '_sitebuiltins', '_bootlocale', '_locale', 'encodings.cp1252', 'types', 'importlib', 'importlib._bootstrap', 'importlib._bootstrap_external', 'warnings', 'importlib.util', 'importlib.abc', 'importlib.machinery', 'contextlib', 'collections', 'operator', '_operator', 'keyword', 'heapq', '_heapq', 'itertools', 'reprlib', '_collections', 'functools', '_functools', 'google', 'mpl_toolkits', 'sphinxcontrib', 'encodings.cp437', 'runpy', 'pkgutil', 'weakref', '_weakrefset', 'main', 're', 'enum', 'sre_compile', '_sre', 'sre_parse', 'sre_constants', 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'tensorflow.keras.applications.nasnet', 'tensorflow.keras.applications.resnet', 'tensorflow.keras.applications.resnet50', 'tensorflow.keras.applications.resnet_v2', 'tensorflow.keras.applications.vgg16', 'tensorflow.keras.applications.vgg19', 'tensorflow.keras.applications.xception', 'tensorflow.keras.backend', 'tensorflow.keras.callbacks', 'tensorflow.keras.callbacks.experimental', 'tensorflow.keras.constraints', 'tensorflow.keras.datasets', 'tensorflow.keras.datasets.boston_housing', 'tensorflow.keras.datasets.cifar10', 'tensorflow.keras.datasets.cifar100', 'tensorflow.keras.datasets.fashion_mnist', 'tensorflow.keras.datasets.imdb', 'tensorflow.keras.datasets.mnist', 'tensorflow.keras.datasets.reuters', 'tensorflow.keras.estimator', 'tensorflow.keras.experimental', 'tensorflow.keras.initializers', 'tensorflow.keras.layers', 'tensorflow.keras.layers.experimental', 'tensorflow.keras.layers.experimental.preprocessing', 'tensorflow.keras.losses', 'tensorflow.keras.metrics', 'tensorflow.keras.mixed_precision', 'tensorflow.keras.mixed_precision.experimental', 'tensorflow.keras.models', 'tensorflow.keras.optimizers', 'tensorflow.keras.optimizers.schedules', 'tensorflow.keras.preprocessing', 'tensorflow.keras.preprocessing.image', 'tensorflow.keras.preprocessing.sequence', 'tensorflow.keras.preprocessing.text', 'tensorflow.keras.regularizers', 'tensorflow.keras.utils', 'tensorflow.keras.wrappers', 'tensorflow.keras.wrappers.scikit_learn', 'cellfinder_core.tools.prep', 'cellfinder_core.tools.tf', 'cellfinder_core.classify', 'cellfinder_core.classify.tools', 'cellfinder_core.classify.resnet', 'cellfinder_core.classify.cube_generator', 'skimage', 'skimage._shared', 'skimage._shared.version_requirements', 'skimage._shared.geometry', 'skimage.util', 'skimage.util.dtype', 'skimage.util.shape', 'skimage.util.noise', 'skimage.util.apply_parallel', 'skimage.util.arraycrop', 'skimage.util.compare', 'skimage.util._regular_grid', 'skimage.util.unique', 'skimage.util._invert', 'skimage.util._montage', 'skimage.util._map_array', 'skimage.util._remap', 'skimage.data', 'skimage.data._binary_blobs', 'skimage.data._registry', 'skimage.util.lookfor', 'skimage.io', 'skimage.io.manage_plugins', 'configparser', 'skimage.io.collection', 'tifffile', 'tifffile.tifffile', 'concurrent.futures.thread', 'skimage.io.sift', 'skimage.io._io', 'skimage.color', 'skimage.color.colorconv', 'skimage.color.colorlabel', 'skimage._shared.utils', 'skimage._shared._warnings', 'skimage.color.rgb_colors', 'skimage.color.delta_e', 'skimage.io.util', 'skimage.exposure', 'skimage.exposure.exposure', 'skimage.exposure._adapthist', 'skimage.color.adapt_rgb', 'skimage.exposure.histogram_matching', 'skimage.io._image_stack', 'skimage.io._plugins', 'skimage.io._plugins.imageio_plugin', 'imageio', 'imageio.core', 'imageio.core.util', 'imageio.core.findlib', 'imageio.core.fetching', 'imageio.core.request', 'imageio.core.format', 'imageio.core.functions', 'imageio.plugins', 'imageio.plugins.tifffile', 'imageio.plugins.pillow', 'imageio.plugins.pillow_info', 'imageio.plugins.pillowmulti', 'imageio.plugins.grab', 'imageio.plugins.freeimage', 'imageio.plugins._freeimage', 'imageio.plugins.freeimagemulti', 'imageio.plugins.ffmpeg', 'imageio.plugins.bsdf', 'imageio.plugins.dicom', 'imageio.plugins.npz', 'imageio.plugins.swf', 'imageio.plugins.feisem', 'imageio.plugins.fits', 'imageio.plugins.simpleitk', 'imageio.plugins.gdal', 'imageio.plugins.lytro', 'imageio.plugins.spe', 'imageio.plugins.example', 'skimage.io._plugins.matplotlib_plugin', 'mpl_toolkits.axes_grid1', 'mpl_toolkits.axes_grid1.axes_size', 'matplotlib', 'matplotlib.cbook', 'matplotlib.cbook.deprecation', 'matplotlib.rcsetup', 'matplotlib.animation', 'matplotlib._animation_data', 'matplotlib.fontconfig_pattern', 'pyparsing', 'matplotlib.colors', 'matplotlib.docstring', 'matplotlib._color_data', 'cycler', 'matplotlib._version', 'matplotlib.ft2font', 'kiwisolver']
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - CACHEDIR=C:\Users\CIBR-ImFacility-WS2.matplotlib
2021-04-28 17:46:25 PM - DEBUG - MainProcess font_manager.py:1436 - Using fontManager instance from C:\Users\CIBR-ImFacility-WS2.matplotlib\fontlist-v330.json
2021-04-28 17:46:25 PM - DEBUG - MainProcess system.py:134 - Determining the maximum number of CPU cores to use
2021-04-28 17:46:25 PM - DEBUG - MainProcess system.py:143 - Number of CPU cores available is: 46
2021-04-28 17:46:25 PM - DEBUG - MainProcess system.py:173 - Setting number of processes to: 46
2021-04-28 17:46:25 PM - DEBUG - MainProcess tf.py:36 - Setting maximum number of threads for tensorflow to: 46
2021-04-28 17:46:26 PM - DEBUG - MainProcess tf.py:26 - No GPUs found, using CPU.
2021-04-28 17:46:26 PM - DEBUG - MainProcess prep.py:53 - No model or weights supplied, so using the default
2021-04-28 17:46:26 PM - DEBUG - MainProcess prep.py:74 - Reading config file: c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\site-packages\cellfinder_core\config\cellfinder.conf.custom
2021-04-28 17:46:26 PM - INFO - MainProcess train_yml.py:345 - Found 155 images from 2 datasets in 1 yaml files
2021-04-28 17:46:26 PM - DEBUG - MainProcess tools.py:37 - Creating a new instance of model: 50-layer
2021-04-28 17:46:27 PM - INFO - MainProcess train_yml.py:361 - Splitting data into training and validation datasets
2021-04-28 17:46:27 PM - INFO - MainProcess train_yml.py:377 - Using 139 images for training and 16 images for validation
2021-04-28 17:46:27 PM - INFO - MainProcess train_yml.py:435 - Beginning training.
Traceback (most recent call last):
File "c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\runpy.py", line 193, in _run_module_as_main
"main", mod_spec)
File "c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\runpy.py", line 85, in _run_code
exec(code, run_globals)
File "C:\Users\CIBR-ImFacility-WS2\Anaconda3\envs\cellfinder\Scripts\cellfinder_train.exe__main.py", line 7, in
(cellfinder) C:\Users\CIBR-ImFacility-WS2>cellfinder_train -y D:\AM_tiff\output\points\training.yml -o D:\AM_tiff\output\points --epochs 100
2021-04-28 17:46:23 PM - INFO - MainProcess fancylog.py:267 - Starting logging
2021-04-28 17:46:23 PM - INFO - MainProcess fancylog.py:269 - Multiprocessing-logging module found. Logging from all processes
2021-04-28 17:46:24 PM - DEBUG - MainProcess tpu_cluster_resolver.py:36 - Falling back to TensorFlow client; we recommended you install the Cloud TPU client directly with pip install cloud-tpu-client.
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - (private) matplotlib data path: c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\site-packages\matplotlib\mpl-data
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - matplotlib data path: c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\site-packages\matplotlib\mpl-data
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - CONFIGDIR=C:\Users\CIBR-ImFacility-WS2.matplotlib
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1480 - matplotlib version 3.3.3
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1481 - interactive is False
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1482 - platform is win32
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:1483 - loaded modules: ['sys', 'builtins', '_frozen_importlib', '_imp', '_thread', '_warnings', '_weakref', 'zipimport', '_frozen_importlib_external', '_io', 'marshal', 'nt', 'winreg', 'encodings', 'codecs', '_codecs', 'encodings.aliases', 'encodings.utf_8', '_signal', 'encodings.latin_1', 'io', 'abc', '_abc', 'site', 'os', 'stat', '_stat', '_collections_abc', 'ntpath', 'genericpath', 'os.path', '_sitebuiltins', '_bootlocale', '_locale', 'encodings.cp1252', 'types', 'importlib', 'importlib._bootstrap', 'importlib._bootstrap_external', 'warnings', 'importlib.util', 'importlib.abc', 'importlib.machinery', 'contextlib', 'collections', 'operator', '_operator', 'keyword', 'heapq', '_heapq', 'itertools', 'reprlib', '_collections', 'functools', '_functools', 'google', 'mpl_toolkits', 'sphinxcontrib', 'encodings.cp437', 'runpy', 'pkgutil', 'weakref', '_weakrefset', 'main', 're', 'enum', 'sre_compile', '_sre', 'sre_parse', 'sre_constants', 'copyreg', 'cellfinder_core', 'cellfinder_core.train', 'cellfinder_core.train.train_yml', 'logging', 'time', 'traceback', 'linecache', 'tokenize', 'token', 'collections.abc', 'string', '_string', 'threading', 'atexit', 'datetime', 'math', '_datetime', 'pathlib', 'fnmatch', 'posixpath', 'errno', 'urllib', 'urllib.parse', 'fancylog', 'fancylog.fancylog', 'fancylog.tools', 'fancylog.tools.git', 'argparse', 'gettext', 'locale', 'sklearn', 'sklearn._config', 'sklearn._distributor_init', 'ctypes', '_ctypes', 'struct', '_struct', 'ctypes._endian', 'sklearn.check_build', 'sklearn.__check_build._check_build', 'cython_runtime', 'sklearn.base', 'copy', 'platform', 'subprocess', 'signal', 'msvcrt', '_winapi', 'inspect', 'dis', 'opcode', '_opcode', 'numpy', 'numpy._globals', 'numpy.config', 'numpy.version', 'numpy._distributor_init', 'glob', 'numpy.core', 'numpy.core.multiarray', 'numpy.core.overrides', 'textwrap', 'numpy.core._multiarray_umath', 'numpy.compat', 'numpy.compat._inspect', 'numpy.compat.py3k', 'pickle', '_compat_pickle', '_pickle', 'numpy.core.umath', 'numpy.core.numerictypes', 'numbers', 'numpy.core._string_helpers', 'numpy.core._type_aliases', 'numpy.core._dtype', 'numpy.core.numeric', 'numpy.core.shape_base', 'numpy.core._asarray', 'numpy.core.fromnumeric', 'numpy.core._methods', 'numpy.core._exceptions', 'numpy.core._ufunc_config', 'numpy.core.arrayprint', 'numpy.core.defchararray', 'numpy.core.records', 'numpy.core.memmap', 'numpy.core.function_base', 'numpy.core.machar', 'numpy.core.getlimits', 'numpy.core.einsumfunc', 'numpy.core._add_newdocs', 'numpy.core._multiarray_tests', 'numpy.core._dtype_ctypes', 'numpy.core._internal', 'ast', '_ast', 'numpy._pytesttester', 'numpy.lib', 'numpy.lib.mixins', 'numpy.lib.scimath', 'numpy.lib.type_check', 'numpy.lib.ufunclike', 'numpy.lib.index_tricks', 'numpy.matrixlib', 'numpy.matrixlib.defmatrix', 'numpy.linalg', 'numpy.linalg.linalg', 'numpy.lib.twodim_base', 'numpy.linalg.lapack_lite', 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'tensorflow.keras.mixed_precision', 'tensorflow.keras.mixed_precision.experimental', 'tensorflow.keras.models', 'tensorflow.keras.optimizers', 'tensorflow.keras.optimizers.schedules', 'tensorflow.keras.preprocessing', 'tensorflow.keras.preprocessing.image', 'tensorflow.keras.preprocessing.sequence', 'tensorflow.keras.preprocessing.text', 'tensorflow.keras.regularizers', 'tensorflow.keras.utils', 'tensorflow.keras.wrappers', 'tensorflow.keras.wrappers.scikit_learn', 'cellfinder_core.tools.prep', 'cellfinder_core.tools.tf', 'cellfinder_core.classify', 'cellfinder_core.classify.tools', 'cellfinder_core.classify.resnet', 'cellfinder_core.classify.cube_generator', 'skimage', 'skimage._shared', 'skimage._shared.version_requirements', 'skimage._shared.geometry', 'skimage.util', 'skimage.util.dtype', 'skimage.util.shape', 'skimage.util.noise', 'skimage.util.apply_parallel', 'skimage.util.arraycrop', 'skimage.util.compare', 'skimage.util._regular_grid', 'skimage.util.unique', 'skimage.util._invert', 'skimage.util._montage', 'skimage.util._map_array', 'skimage.util._remap', 'skimage.data', 'skimage.data._binary_blobs', 'skimage.data._registry', 'skimage.util.lookfor', 'skimage.io', 'skimage.io.manage_plugins', 'configparser', 'skimage.io.collection', 'tifffile', 'tifffile.tifffile', 'concurrent.futures.thread', 'skimage.io.sift', 'skimage.io._io', 'skimage.color', 'skimage.color.colorconv', 'skimage.color.colorlabel', 'skimage._shared.utils', 'skimage._shared._warnings', 'skimage.color.rgb_colors', 'skimage.color.delta_e', 'skimage.io.util', 'skimage.exposure', 'skimage.exposure.exposure', 'skimage.exposure._adapthist', 'skimage.color.adapt_rgb', 'skimage.exposure.histogram_matching', 'skimage.io._image_stack', 'skimage.io._plugins', 'skimage.io._plugins.imageio_plugin', 'imageio', 'imageio.core', 'imageio.core.util', 'imageio.core.findlib', 'imageio.core.fetching', 'imageio.core.request', 'imageio.core.format', 'imageio.core.functions', 'imageio.plugins', 'imageio.plugins.tifffile', 'imageio.plugins.pillow', 'imageio.plugins.pillow_info', 'imageio.plugins.pillowmulti', 'imageio.plugins.grab', 'imageio.plugins.freeimage', 'imageio.plugins._freeimage', 'imageio.plugins.freeimagemulti', 'imageio.plugins.ffmpeg', 'imageio.plugins.bsdf', 'imageio.plugins.dicom', 'imageio.plugins.npz', 'imageio.plugins.swf', 'imageio.plugins.feisem', 'imageio.plugins.fits', 'imageio.plugins.simpleitk', 'imageio.plugins.gdal', 'imageio.plugins.lytro', 'imageio.plugins.spe', 'imageio.plugins.example', 'skimage.io._plugins.matplotlib_plugin', 'mpl_toolkits.axes_grid1', 'mpl_toolkits.axes_grid1.axes_size', 'matplotlib', 'matplotlib.cbook', 'matplotlib.cbook.deprecation', 'matplotlib.rcsetup', 'matplotlib.animation', 'matplotlib._animation_data', 'matplotlib.fontconfig_pattern', 'pyparsing', 'matplotlib.colors', 'matplotlib.docstring', 'matplotlib._color_data', 'cycler', 'matplotlib._version', 'matplotlib.ft2font', 'kiwisolver']
2021-04-28 17:46:25 PM - DEBUG - MainProcess init.py:241 - CACHEDIR=C:\Users\CIBR-ImFacility-WS2.matplotlib
2021-04-28 17:46:25 PM - DEBUG - MainProcess font_manager.py:1436 - Using fontManager instance from C:\Users\CIBR-ImFacility-WS2.matplotlib\fontlist-v330.json
2021-04-28 17:46:25 PM - DEBUG - MainProcess system.py:134 - Determining the maximum number of CPU cores to use
2021-04-28 17:46:25 PM - DEBUG - MainProcess system.py:143 - Number of CPU cores available is: 46
2021-04-28 17:46:25 PM - DEBUG - MainProcess system.py:173 - Setting number of processes to: 46
2021-04-28 17:46:25 PM - DEBUG - MainProcess tf.py:36 - Setting maximum number of threads for tensorflow to: 46
2021-04-28 17:46:26 PM - DEBUG - MainProcess tf.py:26 - No GPUs found, using CPU.
2021-04-28 17:46:26 PM - DEBUG - MainProcess prep.py:53 - No model or weights supplied, so using the default
2021-04-28 17:46:26 PM - DEBUG - MainProcess prep.py:74 - Reading config file: c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\site-packages\cellfinder_core\config\cellfinder.conf.custom
2021-04-28 17:46:26 PM - INFO - MainProcess train_yml.py:345 - Found 155 images from 2 datasets in 1 yaml files
2021-04-28 17:46:26 PM - DEBUG - MainProcess tools.py:37 - Creating a new instance of model: 50-layer
2021-04-28 17:46:27 PM - INFO - MainProcess train_yml.py:361 - Splitting data into training and validation datasets
2021-04-28 17:46:27 PM - INFO - MainProcess train_yml.py:377 - Using 139 images for training and 16 images for validation
2021-04-28 17:46:27 PM - INFO - MainProcess train_yml.py:435 - Beginning training.
Traceback (most recent call last):
File "c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\runpy.py", line 193, in _run_module_as_main
"main", mod_spec)
File "c:\users\cibr-imfacility-ws2\anaconda3\envs\cellfinder\lib\runpy.py", line 85, in _run_code
exec(code, run_globals)
File "C:\Users\CIBR-ImFacility-WS2\Anaconda3\envs\cellfinder\Scripts\cellfinder_train.exe__main.py", line 7, in
The training script is trying to find files in this directory D:\AM_tiff\output\points\non_cells
. Is this directory accessible on your computer? Have you moved the training data, or the yml file?
no, I didnt move any files. but there are none of 'ch1'files in the non-cells folder, and all of them are 'ch0' files.so what
s the definition of the 'ch1' files ? thank you
The ch1 files are the background channel, (ch0 is the signal channel), so there's something wrong with the training data. Do you have any other training data you could test with?
maybe I know whats the problem. Actually, I have two signal channels without reference channel, one of them has few signal point, so I pretend this channel to the background channel, I
m not sure whether it work.
It may not work very well, but it shouldn't cause this bug. Have you managed to train the network with another training dataset?
Are you still having this problem?
I reinstalled the version 0.4.13 rc0, now it can run.
Great, thanks for letting me know. The final 0.4.13 release should be soon.
sorry for the unclearly express, I mean after installed the rc version, there is no error like unrecognized the cellfinder command,but the cellfinder_train still has the error message like I mentioned above
Have you managed to train with another dataset?
do you mean the other raw data or different slices of the same brain dataset?
actually I`ve known the reason why it failed. the background and the signal file number are different. sorry about my carelessness
Describe the bug when I entered the command 'cellfinder_train -y D:\AM_tiff\output\points\training.yml -o D:\AM_tiff\output\points' there is an error ''cellfinder_train' is not recognized as an internal or external command, operable program or batch file.'
I`ve used the cellfinder_curate to produce the .yml profile, so the error like this is so weird. now the version of cellfinder is 0.4.12. hope for your answer. thank you!
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Expected behavior A clear and concise description of what you expected to happen.
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output/cellfinder_2019-12-17_13-29-46.log
)Screenshots If applicable, add screenshots to help explain your problem.
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