This Google Colab Notebook is intended to be used to speed the processing and identification of target gRNA sequences for introduction to Candida species via CRISPR techniques.
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When I'm using the results of this workbook, I'll be taking my selected gRNA sequences into a different program. How can we make this go faster? #3
When using the results of this notebook, I'll be scanning for gRNA sequences that have and MFE of 0, and have a relatively high Total score, then take those results into another program. When I do that I'll need to select the gRNA sequence without the PAM, but include any G or A prefix.
Possible suggestions:
include a column with a checkbox and a button that I can use to generate a simple list of gRNA sequences in those selected rows, but without the PAM
make the value of the gRNA sequence a link that will copy just the portion of the sequence to the clipboard so that I can manually paste it anywhere else.
When using the results of this notebook, I'll be scanning for gRNA sequences that have and MFE of 0, and have a relatively high Total score, then take those results into another program. When I do that I'll need to select the gRNA sequence without the PAM, but include any G or A prefix.
Possible suggestions: