Hi
I am trying to run methylcode and while running it creates the bowtie index for *.fr.c2t.fa(human) that time it fails to create the index file required.
i get this error message
Error: Reference sequence has more than 2^32-1 characters! Please divide the
reference into batches or chunks of about 3.6 billion characters or less each
and index each independently.
I tried using 64 bit version of bowtie but it didn't help, do you know what i doing incorrectly or how should i solve this
du -hs *.fr.c2t.fa
6.5G which is double the size of human reference genome hg19.
Hi I am trying to run methylcode and while running it creates the bowtie index for *.fr.c2t.fa(human) that time it fails to create the index file required.
i get this error message Error: Reference sequence has more than 2^32-1 characters! Please divide the reference into batches or chunks of about 3.6 billion characters or less each and index each independently.
I tried using 64 bit version of bowtie but it didn't help, do you know what i doing incorrectly or how should i solve this
du -hs *.fr.c2t.fa 6.5G which is double the size of human reference genome hg19.
Thanks
Saurabh