In cases where crispr sequences are being searched in sequence-seq, where the same gene sequence is present in the genome -genome, output should reflect handling exact (23 bp) matches.
Since same gene sequence is searched for crisprs, there will be exact matches in the genome for each crispr sequence found. We want to base sorting on 2nd most matched bp in this case and omit exact matches.
In cases where crispr sequences are being searched in sequence
-seq, where the same gene sequence is present in the genome-genome, output should reflect handling exact (23 bp) matches.Since same gene sequence is searched for crisprs, there will be exact matches in the genome for each crispr sequence found. We want to base sorting on 2nd most matched bp in this case and omit exact matches.