RELION shift handling may be incorrect

brisvag / blik

Python tool for visualising and interacting with cryo-ET and subtomogram averaging data.

https://brisvag.github.io/blik/

GNU General Public License v3.0

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RELION shift handling may be incorrect #83

Closed alisterburt closed 3 years ago

alisterburt commented 3 years ago

RELION shifts are currently applied as rlnCoordinateXYZ + rlnOriginXYZ, it's possible that they should be applied as rlnCoordinateXYZ - rlnOriginXYZ, will confirm when I know more

brisvag commented 3 years ago

How do you propose to debug this?

alisterburt commented 3 years ago

It’s debugged and needs inverting (to rlnCoordinateX/Y/X - rlnOriginX/Y/Z) :)

brisvag commented 3 years ago

Lol, ok :P I didn't have any data at hand for which it would be obvious.