Hello. I am currently analyzing single nuclei RNA seq data.
First of all, thank you for developing the great Cell Bender tool.
I am using Cell Bender to remove doublets from my data.
I have a few questions.
After running Cell Bender, I was able to confirm that the number of cells is the same when the output file is loaded in R, but the number of features has decreased. Is this a normal result?
And when I loaded the Cell Bender output file and drew a Vln plot, I was able to confirm that many cells have a nCount_RNA value of 0. Is this okay? Is this a question in the same context as (1)?
Finally, where can I see the description of the figures in the pdf file of the Cell Bender output file?
I am still a beginner, so I have many questions.
I would be grateful if you could give me even a small comment that could be a hint.
Hello. I am currently analyzing single nuclei RNA seq data.
First of all, thank you for developing the great Cell Bender tool.
I am using Cell Bender to remove doublets from my data.
I have a few questions.
After running Cell Bender, I was able to confirm that the number of cells is the same when the output file is loaded in R, but the number of features has decreased. Is this a normal result?
And when I loaded the Cell Bender output file and drew a Vln plot, I was able to confirm that many cells have a nCount_RNA value of 0. Is this okay? Is this a question in the same context as (1)?
Finally, where can I see the description of the figures in the pdf file of the Cell Bender output file?
I am still a beginner, so I have many questions.
I would be grateful if you could give me even a small comment that could be a hint.
Thank you in advance.