Our group is attempting to trial different protocols for single nuclei 10x experiments from different tissues. I have run cellbender and I have gotten the inference result below. I am wondering how to intepret this. In the past I have gotten much cleaner results from cellbender when looking at cells (PMBCs) and I'm wondering if there's any parameters I could try tweaking in cellbender to make it more amenable to nuclei if that is indeed the issue.
Hi,
Our group is attempting to trial different protocols for single nuclei 10x experiments from different tissues. I have run cellbender and I have gotten the inference result below. I am wondering how to intepret this. In the past I have gotten much cleaner results from cellbender when looking at cells (PMBCs) and I'm wondering if there's any parameters I could try tweaking in cellbender to make it more amenable to nuclei if that is indeed the issue.
Thank you a bunch for your help here!