Open najibveto opened 8 months ago
The command that you run should not contain the square brackets, [
and ]
; these indicate what you should fill in. For instance, in your example, the command you run could be:
design.py complete-targets fasta INHV_N.fasta -o out
Sorry for the confusion about this in the README.
The examples section in the README has additional examples without the square brackets.
The command that you run should not contain the square brackets,
[
and]
; these indicate what you should fill in. For instance, in your example, the command you run could be:design.py complete-targets fasta INHV_N.fasta -o out
Sorry for the confusion about this in the README.
The examples section in the README has additional examples without the square brackets.
thank a lot for yoru reply. just one question if possible, i run the command and i got the following message:
Traceback (most recent call last):
File "/home/najib/miniconda3/envs/adapt/bin/design.py", line 1728, in <module>
run(argv_to_args(sys.argv))
File "/home/najib/miniconda3/envs/adapt/bin/design.py", line 970, in run
design_for_id(args)
File "/home/najib/miniconda3/envs/adapt/bin/design.py", line 756, in design_for_id
raise Exception(("Either --predict-activity-model-path or "
Exception: Either --predict-activity-model-path or --predict-cas13a-activity-model must be specified if --obj is 'maximize-activity'. Alternatively, if you cannot use our pre-trained Cas13a model nor another model, you can set --use-simple-binary-activity-prediction
i run it again and i got the results. i am curious should i opt for pre-trained Cas13a and can give better accuracy?. thank you for your help.
I suggest using the model, by specifying --predict-cas13a-activity-model
, if and only if the designs are to be used for RNA detection with LwaCas13a. Otherwise, you could specify --use-simple-binary-activity-prediction
to instead use a simple mismatch and PFS-based model for the crRNAs.
I suggest using the model, by specifying
--predict-cas13a-activity-model
, if and only if the designs are to be used for RNA detection with LwaCas13a. Otherwise, you could specify--use-simple-binary-activity-prediction
to instead use a simple mismatch and PFS-based model for the crRNAs.
thank you a lot for your help.
hello, i am trying to run the following command:
design.py [complete-targets] fasta [INHV_N.fasta] -o [out-tsv]
however i got the following error:i would like to know what is the correct command for doing it. thank you for your help.