broadinstitute / adapt

A package for designing activity-informed nucleic acid diagnostics for viruses.
MIT License
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Add option to require flanking sequence (PFS) on guides #9

Closed haydenm closed 5 years ago

haydenm commented 5 years ago

This adds the arguments --require-flanking5 and --require-flanking3 to design_guides.py. When set, these require a particular sequence on the 5'/3' protospacer flanking sites (PFS) of a guide in the design of guides.