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broadinstitute / cell-health

Predicting Cell Health with Morphological Profiles
MIT License
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From this experiment, `ITGAV` and `MYC` are the highest correlating genes across guides #14

Closed gwaybio closed 5 years ago

gwaybio commented 5 years ago

A question arose about selecting CRISPR guides to use as positive controls in a cell painting experiment.

Based on the limited data:

guide_correlation

it appears that the most reproducible profiles are from CRISPR guides targeting ITGAV, KIF11, MYC, POLR2D, and PSMA1.

I generated a morpheus heatmap comparing aggregate correlation (calculating mean well profiles) for these genes (and a negative control LacZ).

crispr_reproducible_genes

The results seem to indicate that many of these reproducible profiles across guides are also highly similar within cell line.

gwaybio commented 4 years ago

Also see #7 for generating replicate correlation analysis figure