In the Chronos 1 paper, non-expressed genes are used to define the negative control sgRNAs for each line. Is there a way to do the same in Chronos 2? As best as I can tell, that can only be done on a per-library basis.
Some sub-genome libraries may only have 25-100 non-targetting sgRNAs as negative controls, which throw an error in Chronos if <100 sgRNAs are defined. Being able to supplement/replace them with the cutting sgRNAs for non-expressed genes in each line is enticing.
In the Chronos 1 paper, non-expressed genes are used to define the negative control sgRNAs for each line. Is there a way to do the same in Chronos 2? As best as I can tell, that can only be done on a per-library basis.
Some sub-genome libraries may only have 25-100 non-targetting sgRNAs as negative controls, which throw an error in Chronos if <100 sgRNAs are defined. Being able to supplement/replace them with the cutting sgRNAs for non-expressed genes in each line is enticing.