I'm trying to run this pipeline using v10 on a quant-seq library (stranded fr), but the *.gene_reads.gct output file contains only zeros for column 3 (counts).
I'm building a STAR index using gencode v30 along with the indicated genome ref (grch38):
Then running STAR alignment, marking dups with Picard, and then running RNA-SeQC with the gencode.v30.*.collapsed_only.gtf like so (and note that I checked the .md.bam file, and it does contain many high quality alignments):
I'm trying to run this pipeline using v10 on a quant-seq library (stranded fr), but the
*.gene_reads.gct
output file contains only zeros for column 3 (counts).I'm building a STAR index using gencode v30 along with the indicated genome ref (grch38):
Then running STAR alignment, marking dups with Picard, and then running RNA-SeQC with the
gencode.v30.*.collapsed_only.gtf
like so (and note that I checked the.md.bam
file, and it does contain many high quality alignments):The only error I'm getting is tracing back to gzip, so I don't think that's the issue:
Any suggestions, or anything obviously out of wack with the above?
Thanks!