Hello, I collected some blood samples from cancer patients to pull-down 5hmC-containing cfDNA fragments for sequencing (PE150, coverage is about 1X), which are different from cfDNA-WGS data in the paper. I wonder if the cutoff of 0.03 is appropriate for these data and if there are some aspects I need to pay attention to while processing cfDNA 5hmC data with ichorCNA. Thank you very much for any advice !
Hello, I collected some blood samples from cancer patients to pull-down 5hmC-containing cfDNA fragments for sequencing (PE150, coverage is about 1X), which are different from cfDNA-WGS data in the paper. I wonder if the cutoff of 0.03 is appropriate for these data and if there are some aspects I need to pay attention to while processing cfDNA 5hmC data with ichorCNA. Thank you very much for any advice !