brianjohnhaas
commented
7 months ago Hi,
You can experiment with it, but we generally only do single sample normalizations using normal cells sequenced within the tumor samples as the reference.
On Fri, Mar 1, 2024 at 12:23 PM Zhichao Wu @.***> wrote:
Hi, I did not find the answer to this question. And I am not sure if this is something reasonable to do. Can I use single cell data generated by SmartSeq as a normal reference to infer CNVs on 10x genomics single-cell sequencing? Vice versa, is it possible to use single-cell data generated by 10x genomics as a normal reference to infer CNVs on SmartSeq data?
Thank you!
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Brian J. Haas The Broad Institute http://broadinstitute.org/~bhaas http://broad.mit.edu/~bhaas
Hi, I did not find the answer to this question. And I am not sure if this is something reasonable to do. Can I use single cell data generated by SmartSeq as a normal reference to infer CNVs on 10x genomics single-cell sequencing? Vice versa, is it possible to use single-cell data generated by 10x genomics as a normal reference to infer CNVs on SmartSeq data?
Thank you!